TY - JOUR
T1 - Fatty acids as modulators of membrane functions
T2 - catecholamine activated adenylate cyclase of the turkey erythrocyte
AU - Orly, J.
AU - Schramm, M.
PY - 1975
Y1 - 1975
N2 - Activation of the adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] from turkey erythrocytes by isoproterenol decreased precipitously below 26°. Certain unsaturated fatty acids enhanced the activation by isoproterenol up to 25 fold at reduced temperatures. The fatty acid also enhanced the formation of a persistent active state of the enzyme which was produced by preincubation with guanosine 5' (β,γ imino)triphosphate [Gpp(NH)p]. Once the enzyme had been activated by Gpp(NH)p plus isoproterenol the reaction rate was no longer as temperature sensitive and the fatty acid had little effect. The synthetic Gpp(NH)p apparently substituted for the natural GTP, which is known to play a regulatory role in the adenylate cyclase system. The findings suggest that the function of GTP which is mediated by the hormone is the temperature sensitive event which is enhanced by the fatty acid. The use of free acid to probe membrane associated reactions in intact cells and in isolated membrane preparations is proposed.
AB - Activation of the adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] from turkey erythrocytes by isoproterenol decreased precipitously below 26°. Certain unsaturated fatty acids enhanced the activation by isoproterenol up to 25 fold at reduced temperatures. The fatty acid also enhanced the formation of a persistent active state of the enzyme which was produced by preincubation with guanosine 5' (β,γ imino)triphosphate [Gpp(NH)p]. Once the enzyme had been activated by Gpp(NH)p plus isoproterenol the reaction rate was no longer as temperature sensitive and the fatty acid had little effect. The synthetic Gpp(NH)p apparently substituted for the natural GTP, which is known to play a regulatory role in the adenylate cyclase system. The findings suggest that the function of GTP which is mediated by the hormone is the temperature sensitive event which is enhanced by the fatty acid. The use of free acid to probe membrane associated reactions in intact cells and in isolated membrane preparations is proposed.
UR - http://www.scopus.com/inward/record.url?scp=0011168515&partnerID=8YFLogxK
U2 - 10.1073/pnas.72.9.3433
DO - 10.1073/pnas.72.9.3433
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 1059128
AN - SCOPUS:0011168515
SN - 0027-8424
VL - 72
SP - 3433
EP - 3437
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9
ER -