Abstract
The mounting evidence for the involvement of astrocytes in neuronal circuits function and behavior stands in stark contrast to the lack of detailed anatomical description of these cells and the neurons in their domains. To fill this void, we imaged >30,000 astrocytes in hippocampi made transparent by CLARITY, and determined the elaborate structure, distribution, and neuronal content of astrocytic domains. First, we characterized the spatial distribution of >19,000 astrocytes across CA1 lamina, and analyzed the morphology of thousands of reconstructed domains. We then determined the excitatory somatic content of CA1 astrocytes, and measured the distance between inhibitory neuronal somata to the nearest astrocyte soma. We find that on average, there are almost 14 pyramidal neurons per domain in the CA1, increasing toward the pyramidal layer midline, compared to only five excitatory neurons per domain in the amygdala. Finally, we discovered that somatostatin neurons are found in close proximity to astrocytes, compared to parvalbumin and VIP inhibitory neurons. This work provides a comprehensive large-scale quantitative foundation for studying neuron-astrocyte interactions.
| Original language | American English |
|---|---|
| Pages (from-to) | 2378-2390 |
| Number of pages | 13 |
| Journal | GLIA |
| Volume | 69 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2021 |
Bibliographical note
Funding Information:The authors thank the entire Goshen lab for their support. AD is supported by the Azrieli fellowship and the ELSC graduate students scholarship. This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No 803589), the Israel Science Foundation (ISF grant No. 1815/18), and the Canada-Israel grants (CIHR-ISF, grant No. 2591/18). The authors thank Ami Citri and Adi Kol for the critical reading of the manuscript.
Funding Information:
The authors thank the entire Goshen lab for their support. AD is supported by the Azrieli fellowship and the ELSC graduate students scholarship. This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No 803589), the Israel Science Foundation (ISF grant No. 1815/18), and the Canada‐Israel grants (CIHR‐ISF, grant No. 2591/18). The authors thank Ami Citri and Adi Kol for the critical reading of the manuscript.
Funding Information:
H2020 European Research Council, Grant/Award Number: 803589; Israel Science Foundation, Grant/Award Numbers: 1815/18, 2591/18 Funding information
Publisher Copyright:
© 2021 Wiley Periodicals LLC.
Keywords
- CLARITY
- VIP
- amygdala
- hippocampus
- parvalbumin
- pyramidal neurons
- somatostatin neurons