FeDFOB and FeEDDHA immobilized on Sepharose gels as an Fe sources to plants

Zehava Yehuda, Yitzhak Hadar, Yona Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Background and Aims: The idea of immobilizing Fe and the formation of a slow-release fertilizer has been considered in several studies. However, none of the proposed materials were found efficient in correcting Fe deficiency. In the present study immobilization of Fe chelates on a solid matrix, as a first step in the production and supply process, is tested. Methods: The free forms of the siderophores desferrioxamine B (DFOB) and ethylendiaminebis(o-hydroxyphenyl-acetic acid) (EDDHA) were immobilized on p-nitrophenylchloroformate activated Sepharose and tested as Fe source for both strategy I (peanuts and cucumber, Arachis hypogeae L. and Cucumis sativus L., respectively) and strategy II (barley and wheat, Hordeum vulgare L. and Triticum aestivum L., respectively), plants. Plants grown in hydroponic cultures at pH 7. 3 were supplied with the immobilized Fe-chelate either free in solution or confined in a dialysis tube with or without EDDHA in the outer solution as a carrier. Results: Cucumber and barley plants were found efficient in acquiring Fe from the immobilized chelates. Conclusions: The effectiveness in utilizing the immobilized Fe-chelate differed among cultivars and was related to the efficiency of the reductases activity or phytosiderophores secretion systems, in Strategy I and Strategy II plants, respectively.

Original languageEnglish
Pages (from-to)379-391
Number of pages13
JournalPlant and Soil
Volume350
Issue number1-2
DOIs
StatePublished - Jan 2012

Keywords

  • Desferrioxamine B (DFOB)
  • Ethylendiaminebis(o-hydroxyphenyl-acetic acid) (EDDHA)
  • Fe chelators
  • Immobilized chelates
  • Phytosiderophores
  • Reduction

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