Female-specific DNA sequences in the chicken genome

Zur Granevitze, Shula Blum, Hans Cheng, Alain Vignal, Mireille Morisson, Giora Ben-Ari, Lior David, Marcus William Feldman, Steffen Weigend, Jossi Hillel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks.

Original languageAmerican English
Pages (from-to)238-242
Number of pages5
JournalJournal of Heredity
Issue number3
StatePublished - May 2007


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