Female-specific DNA sequences in the chicken genome

Zur Granevitze; Shula Blum; Hans Cheng; Alain Vignal; Mireille Morisson; Giora Ben-Ari; Lior David; Marcus William Feldman; Steffen Weigend; Jossi Hillel

doi:10.1093/jhered/esm010

Female-specific DNA sequences in the chicken genome

Zur Granevitze
, Shula Blum
, Hans Cheng
, Alain Vignal
, Mireille Morisson
, Giora Ben-Ari
, Lior David
, Marcus William Feldman
, Steffen Weigend
, Jossi Hillel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks.

Original language	English
Pages (from-to)	238-242
Number of pages	5
Journal	Journal of Heredity
Volume	98
Issue number	3
DOIs	https://doi.org/10.1093/jhered/esm010
State	Published - May 2007

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10.1093/jhered/esm010

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title = "Female-specific DNA sequences in the chicken genome",

abstract = "Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks.",

author = "Zur Granevitze and Shula Blum and Hans Cheng and Alain Vignal and Mireille Morisson and Giora Ben-Ari and Lior David and Feldman, \{Marcus William\} and Steffen Weigend and Jossi Hillel",

year = "2007",

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doi = "10.1093/jhered/esm010",

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T1 - Female-specific DNA sequences in the chicken genome

AU - Granevitze, Zur

AU - Blum, Shula

AU - Cheng, Hans

AU - Vignal, Alain

AU - Morisson, Mireille

AU - Ben-Ari, Giora

AU - David, Lior

AU - Feldman, Marcus William

AU - Weigend, Steffen

AU - Hillel, Jossi

PY - 2007/5

Y1 - 2007/5

N2 - Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks.

AB - Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks.

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VL - 98

SP - 238

EP - 242

JO - Journal of Heredity

JF - Journal of Heredity

IS - 3

ER -

Female-specific DNA sequences in the chicken genome

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