TY - JOUR
T1 - Fhit delocalizes Annexin A4 from plasma membrane to cytosol and sensitizes lung cancer cells to paclitaxel
AU - Gaudio, Eugenio
AU - Paduano, Francesco
AU - Spizzo, Riccardo
AU - Ngankeu, Apollinaire
AU - Zanesi, Nicola
AU - Gaspari, Marco
AU - Ortuso, Francesco
AU - Lovat, Francesca
AU - Rock, Jonathan
AU - Hill, Grace A.
AU - Kaou, Mohamed
AU - Cuda, Giovanni
AU - Aqeilan, Rami I.
AU - Alcaro, Stefano
AU - Croce, Carlo M.
AU - Trapasso, Francesco
PY - 2013/11/6
Y1 - 2013/11/6
N2 - Fhit protein is lost or reduced in a large fraction of human tumors, and its restoration triggers apoptosis and suppresses tumor formation or progression in preclinical models. Here, we describe the identification of candidate Fhit-interacting proteins with cytosolic and plasma membrane localization. Among these, Annexin 4 (ANXA4) was validated by co-immunoprecipitation and confocal microscopy as a partner of this novel Fhit protein complex. Here we report that overexpression of Fhit prevents Annexin A4 translocation from cytosol to plasma membrane in A549 lung cancer cells treated with paclitaxel. Moreover, paclitaxel administration in combination with AdFHIT acts synergistically to increase the apoptotic rate of tumor cells both in vitro and in vivo experiments.
AB - Fhit protein is lost or reduced in a large fraction of human tumors, and its restoration triggers apoptosis and suppresses tumor formation or progression in preclinical models. Here, we describe the identification of candidate Fhit-interacting proteins with cytosolic and plasma membrane localization. Among these, Annexin 4 (ANXA4) was validated by co-immunoprecipitation and confocal microscopy as a partner of this novel Fhit protein complex. Here we report that overexpression of Fhit prevents Annexin A4 translocation from cytosol to plasma membrane in A549 lung cancer cells treated with paclitaxel. Moreover, paclitaxel administration in combination with AdFHIT acts synergistically to increase the apoptotic rate of tumor cells both in vitro and in vivo experiments.
UR - http://www.scopus.com/inward/record.url?scp=84892381951&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0078610
DO - 10.1371/journal.pone.0078610
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 24223161
AN - SCOPUS:84892381951
SN - 1932-6203
VL - 8
JO - PLoS ONE
JF - PLoS ONE
IS - 11
M1 - e78610
ER -