Fibrinogen coagulation without thrombin: Reaction with vitamin C and copper(II)

Gerard Marx*, Mordechai Chevion

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We describe a novel method for inducing fibrinogen derived clots. The addition of vitamin C (0.1-1 mM) to a solution of fibrinogen (1 mg/ml) and Cu(II) (20-150 μM) results in protein coming out of solution . This phenomenon can be "read" by fibrometers as "clotting time". The reaction requires Cu(II) and can be prevented by a chelating agent, such as citrate, as well by a hydroxyl radical scavenger, such as mannitol. The insoluble protein, called "neofibe", is soluble in 4 M urea and 2 % SDS. Isoelectric focusing and SDS-electrophoretic comparison of native fibrinogen with neofibe reveal molecular modifications of the starting protein. This reaction is an interesting example of a free radical mediated transformation of soluble protein into insoluble material . Some findings on the connection between hemostasis, vitamin C and Cu(II) are discussed.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalThrombosis Research
Volume40
Issue number1
DOIs
StatePublished - 1 Oct 1985

Keywords

  • Chain breaks
  • Free radicals
  • Protein modification

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