TY - JOUR
T1 - Fibrinogen coagulation without thrombin
T2 - Reaction with vitamin C and copper(II)
AU - Marx, Gerard
AU - Chevion, Mordechai
PY - 1985/10/1
Y1 - 1985/10/1
N2 - We describe a novel method for inducing fibrinogen derived clots. The addition of vitamin C (0.1-1 mM) to a solution of fibrinogen (1 mg/ml) and Cu(II) (20-150 μM) results in protein coming out of solution . This phenomenon can be "read" by fibrometers as "clotting time". The reaction requires Cu(II) and can be prevented by a chelating agent, such as citrate, as well by a hydroxyl radical scavenger, such as mannitol. The insoluble protein, called "neofibe", is soluble in 4 M urea and 2 % SDS. Isoelectric focusing and SDS-electrophoretic comparison of native fibrinogen with neofibe reveal molecular modifications of the starting protein. This reaction is an interesting example of a free radical mediated transformation of soluble protein into insoluble material . Some findings on the connection between hemostasis, vitamin C and Cu(II) are discussed.
AB - We describe a novel method for inducing fibrinogen derived clots. The addition of vitamin C (0.1-1 mM) to a solution of fibrinogen (1 mg/ml) and Cu(II) (20-150 μM) results in protein coming out of solution . This phenomenon can be "read" by fibrometers as "clotting time". The reaction requires Cu(II) and can be prevented by a chelating agent, such as citrate, as well by a hydroxyl radical scavenger, such as mannitol. The insoluble protein, called "neofibe", is soluble in 4 M urea and 2 % SDS. Isoelectric focusing and SDS-electrophoretic comparison of native fibrinogen with neofibe reveal molecular modifications of the starting protein. This reaction is an interesting example of a free radical mediated transformation of soluble protein into insoluble material . Some findings on the connection between hemostasis, vitamin C and Cu(II) are discussed.
KW - Chain breaks
KW - Free radicals
KW - Protein modification
UR - http://www.scopus.com/inward/record.url?scp=0022341217&partnerID=8YFLogxK
U2 - 10.1016/0049-3848(85)90345-7
DO - 10.1016/0049-3848(85)90345-7
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C2 - 4089822
AN - SCOPUS:0022341217
SN - 0049-3848
VL - 40
SP - 11
EP - 18
JO - Thrombosis Research
JF - Thrombosis Research
IS - 1
ER -