TY - JOUR
T1 - Fine structure and enzymes of matrix vesicles in Osteosarcoma
T2 - Possible occurrence of contractile proteins
AU - Muhlrad, A.
AU - Stein, H.
AU - Bab, I. A.
AU - Sela, J.
PY - 1978
Y1 - 1978
N2 - Identification and isolation of extracellular matrix vesicles were performed from an osteosarcona. They were characterized as trilaminated membrane-bound organelles with diameters ranging between 0.05 - 0.45 μm. The vesicle fraction showed remarkably high alkaline phosphatase, pyrophosphatase and K+ (EDTA) mediated ATPase activities when compared to the membrane and cellular fractions. Occurrence of K+ (EDTA) mediated ATPase indicated the presence of myosin. Electrophoretic bands, corresponding to the contractile proteins, actin and myosin, supported the findings of enzymatic assays.
AB - Identification and isolation of extracellular matrix vesicles were performed from an osteosarcona. They were characterized as trilaminated membrane-bound organelles with diameters ranging between 0.05 - 0.45 μm. The vesicle fraction showed remarkably high alkaline phosphatase, pyrophosphatase and K+ (EDTA) mediated ATPase activities when compared to the membrane and cellular fractions. Occurrence of K+ (EDTA) mediated ATPase indicated the presence of myosin. Electrophoretic bands, corresponding to the contractile proteins, actin and myosin, supported the findings of enzymatic assays.
KW - Alkaline phosphatase
KW - Contractile proteins
KW - Matrix vesicles
KW - Osteosarcoma
UR - http://www.scopus.com/inward/record.url?scp=0342470019&partnerID=8YFLogxK
U2 - 10.1016/0221-8747(78)90007-3
DO - 10.1016/0221-8747(78)90007-3
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AN - SCOPUS:0342470019
SN - 0221-8747
VL - 1
SP - 227
EP - 233
JO - Metabolic Bone Disease and Related Research
JF - Metabolic Bone Disease and Related Research
IS - 3
ER -