Fine structure and enzymes of matrix vesicles in Osteosarcoma: Possible occurrence of contractile proteins

A. Muhlrad, H. Stein, I. A. Bab, J. Sela*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Identification and isolation of extracellular matrix vesicles were performed from an osteosarcona. They were characterized as trilaminated membrane-bound organelles with diameters ranging between 0.05 - 0.45 μm. The vesicle fraction showed remarkably high alkaline phosphatase, pyrophosphatase and K+ (EDTA) mediated ATPase activities when compared to the membrane and cellular fractions. Occurrence of K+ (EDTA) mediated ATPase indicated the presence of myosin. Electrophoretic bands, corresponding to the contractile proteins, actin and myosin, supported the findings of enzymatic assays.

Original languageEnglish
Pages (from-to)227-233
Number of pages7
JournalMetabolic Bone Disease and Related Research
Volume1
Issue number3
DOIs
StatePublished - 1978
Externally publishedYes

Keywords

  • Alkaline phosphatase
  • Contractile proteins
  • Matrix vesicles
  • Osteosarcoma

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