Fine structure and enzymes of matrix vesicles in Osteosarcoma: Possible occurrence of contractile proteins

A. Muhlrad; H. Stein; I. A. Bab; J. Sela

doi:10.1016/0221-8747(78)90007-3

Fine structure and enzymes of matrix vesicles in Osteosarcoma: Possible occurrence of contractile proteins

A. Muhlrad
, H. Stein
, I. A. Bab
, J. Sela^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Identification and isolation of extracellular matrix vesicles were performed from an osteosarcona. They were characterized as trilaminated membrane-bound organelles with diameters ranging between 0.05 - 0.45 μm. The vesicle fraction showed remarkably high alkaline phosphatase, pyrophosphatase and K⁺ (EDTA) mediated ATPase activities when compared to the membrane and cellular fractions. Occurrence of K⁺ (EDTA) mediated ATPase indicated the presence of myosin. Electrophoretic bands, corresponding to the contractile proteins, actin and myosin, supported the findings of enzymatic assays.

Original language	English
Pages (from-to)	227-233
Number of pages	7
Journal	Metabolic Bone Disease and Related Research
Volume	1
Issue number	3
DOIs	https://doi.org/10.1016/0221-8747(78)90007-3
State	Published - 1978
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Alkaline phosphatase
Contractile proteins
Matrix vesicles
Osteosarcoma

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10.1016/0221-8747(78)90007-3

Cite this

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title = "Fine structure and enzymes of matrix vesicles in Osteosarcoma: Possible occurrence of contractile proteins",

abstract = "Identification and isolation of extracellular matrix vesicles were performed from an osteosarcona. They were characterized as trilaminated membrane-bound organelles with diameters ranging between 0.05 - 0.45 μm. The vesicle fraction showed remarkably high alkaline phosphatase, pyrophosphatase and K+ (EDTA) mediated ATPase activities when compared to the membrane and cellular fractions. Occurrence of K+ (EDTA) mediated ATPase indicated the presence of myosin. Electrophoretic bands, corresponding to the contractile proteins, actin and myosin, supported the findings of enzymatic assays.",

keywords = "Alkaline phosphatase, Contractile proteins, Matrix vesicles, Osteosarcoma",

author = "A. Muhlrad and H. Stein and Bab, \{I. A.\} and J. Sela",

year = "1978",

doi = "10.1016/0221-8747(78)90007-3",

language = "אנגלית",

volume = "1",

pages = "227--233",

journal = "Metabolic Bone Disease and Related Research",

issn = "0221-8747",

publisher = "Elsevier Inc.",

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}

TY - JOUR

T1 - Fine structure and enzymes of matrix vesicles in Osteosarcoma

T2 - Possible occurrence of contractile proteins

AU - Muhlrad, A.

AU - Stein, H.

AU - Bab, I. A.

AU - Sela, J.

PY - 1978

Y1 - 1978

N2 - Identification and isolation of extracellular matrix vesicles were performed from an osteosarcona. They were characterized as trilaminated membrane-bound organelles with diameters ranging between 0.05 - 0.45 μm. The vesicle fraction showed remarkably high alkaline phosphatase, pyrophosphatase and K+ (EDTA) mediated ATPase activities when compared to the membrane and cellular fractions. Occurrence of K+ (EDTA) mediated ATPase indicated the presence of myosin. Electrophoretic bands, corresponding to the contractile proteins, actin and myosin, supported the findings of enzymatic assays.

AB - Identification and isolation of extracellular matrix vesicles were performed from an osteosarcona. They were characterized as trilaminated membrane-bound organelles with diameters ranging between 0.05 - 0.45 μm. The vesicle fraction showed remarkably high alkaline phosphatase, pyrophosphatase and K+ (EDTA) mediated ATPase activities when compared to the membrane and cellular fractions. Occurrence of K+ (EDTA) mediated ATPase indicated the presence of myosin. Electrophoretic bands, corresponding to the contractile proteins, actin and myosin, supported the findings of enzymatic assays.

KW - Alkaline phosphatase

KW - Contractile proteins

KW - Matrix vesicles

KW - Osteosarcoma

UR - https://www.scopus.com/pages/publications/0342470019

U2 - 10.1016/0221-8747(78)90007-3

DO - 10.1016/0221-8747(78)90007-3

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AN - SCOPUS:0342470019

SN - 0221-8747

VL - 1

SP - 227

EP - 233

JO - Metabolic Bone Disease and Related Research

JF - Metabolic Bone Disease and Related Research

IS - 3

ER -

Fine structure and enzymes of matrix vesicles in Osteosarcoma: Possible occurrence of contractile proteins

Abstract

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Keywords

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