Flow Cytometry: A New Method to Investigate the Properties of Water-in-Oil-in-Water Emulsions

Mingtan Hai, Kalia Bernath, Dan Tawfik, Shlomo Magdassi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

We present a new and facile method to evaluate w/o/w emulsions containing fluorescent markers by flow cytometry. Flow cytometry allows simultaneous measurement of w/o/w emulsion droplets "marked" with a fluorescent marker or "blank" without the need for complicated sample preparation. The yield of preparation of the w/o/w emulsion and the release rate of the fluorescent marker FITC-BSA were investigated by this new method. The release fraction (after 24 h) of FITC-BSA from the w/o/w emulsion decreased with increasing concentration of FITC-BSA inside the internal phase, just like the release fraction of NaCl as marker from the w/o/w emulsion. Flow cytometry results show that the yield and release behavior in w/o/w emulsions are in agreement with results reported by more complicated methods.

Original languageAmerican English
Pages (from-to)2081-2085
Number of pages5
JournalLangmuir
Volume20
Issue number6
DOIs
StatePublished - 16 Mar 2004

Fingerprint

Dive into the research topics of 'Flow Cytometry: A New Method to Investigate the Properties of Water-in-Oil-in-Water Emulsions'. Together they form a unique fingerprint.

Cite this