Flow Cytometry: A New Method to Investigate the Properties of Water-in-Oil-in-Water Emulsions

Mingtan Hai; Kalia Bernath; Dan Tawfik; Shlomo Magdassi

doi:10.1021/la035402+

Flow Cytometry: A New Method to Investigate the Properties of Water-in-Oil-in-Water Emulsions

Mingtan Hai, Kalia Bernath, Dan Tawfik, Shlomo Magdassi^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

We present a new and facile method to evaluate w/o/w emulsions containing fluorescent markers by flow cytometry. Flow cytometry allows simultaneous measurement of w/o/w emulsion droplets "marked" with a fluorescent marker or "blank" without the need for complicated sample preparation. The yield of preparation of the w/o/w emulsion and the release rate of the fluorescent marker FITC-BSA were investigated by this new method. The release fraction (after 24 h) of FITC-BSA from the w/o/w emulsion decreased with increasing concentration of FITC-BSA inside the internal phase, just like the release fraction of NaCl as marker from the w/o/w emulsion. Flow cytometry results show that the yield and release behavior in w/o/w emulsions are in agreement with results reported by more complicated methods.

Original language	English
Pages (from-to)	2081-2085
Number of pages	5
Journal	Langmuir
Volume	20
Issue number	6
DOIs	https://doi.org/10.1021/la035402+
State	Published - 16 Mar 2004

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T2 - A New Method to Investigate the Properties of Water-in-Oil-in-Water Emulsions

AU - Hai, Mingtan

AU - Bernath, Kalia

AU - Tawfik, Dan

AU - Magdassi, Shlomo

PY - 2004/3/16

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N2 - We present a new and facile method to evaluate w/o/w emulsions containing fluorescent markers by flow cytometry. Flow cytometry allows simultaneous measurement of w/o/w emulsion droplets "marked" with a fluorescent marker or "blank" without the need for complicated sample preparation. The yield of preparation of the w/o/w emulsion and the release rate of the fluorescent marker FITC-BSA were investigated by this new method. The release fraction (after 24 h) of FITC-BSA from the w/o/w emulsion decreased with increasing concentration of FITC-BSA inside the internal phase, just like the release fraction of NaCl as marker from the w/o/w emulsion. Flow cytometry results show that the yield and release behavior in w/o/w emulsions are in agreement with results reported by more complicated methods.

AB - We present a new and facile method to evaluate w/o/w emulsions containing fluorescent markers by flow cytometry. Flow cytometry allows simultaneous measurement of w/o/w emulsion droplets "marked" with a fluorescent marker or "blank" without the need for complicated sample preparation. The yield of preparation of the w/o/w emulsion and the release rate of the fluorescent marker FITC-BSA were investigated by this new method. The release fraction (after 24 h) of FITC-BSA from the w/o/w emulsion decreased with increasing concentration of FITC-BSA inside the internal phase, just like the release fraction of NaCl as marker from the w/o/w emulsion. Flow cytometry results show that the yield and release behavior in w/o/w emulsions are in agreement with results reported by more complicated methods.

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Flow Cytometry: A New Method to Investigate the Properties of Water-in-Oil-in-Water Emulsions

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