Flower proteome: Changes in protein spectrum during the advanced stages of rose petal development

Mery Dafny-Yelin, Inna Guterman, Naama Menda, Mariana Ovadis, Moshe Shalit, Eran Pichersky, Dani Zamir, Efraim Lewinsohn, Zach Adam, David Weiss, Alexander Vainstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Flowering is a unique and highly programmed process, but hardly anything is known about the developmentally regulated proteome changes in petals. Here, we employed proteomic technologies to study petal development in rose (Rosa hybrida). Using two-dimensional polyacrylamide gel electrophoresis, we generated stage-specific (closed bud, mature flower and flower at anthesis) petal protein maps with ca. 1,000 unique protein spots. Expression analyses of all resolved protein spots revealed that almost 30% of them were stage-specific, with ca. 90 protein spots for each stage. Most of the proteins exhibited differential expression during petal development, whereas only ca. 6% were constitutively expressed. Eighty-two of the resolved proteins were identified by mass spectrometry and annotated. Classification of the annotated proteins into functional groups revealed energy, cell rescue, unknown function (including novel sequences) and metabolism to be the largest classes, together comprising ca. 90% of all identified proteins. Interestingly, a large number of stress-related proteins were identified in developing petals. Analyses of the expression patterns of annotated proteins and their corresponding RNAs confirmed the importance of proteome characterization.

Original languageAmerican English
Pages (from-to)37-46
Number of pages10
JournalPlanta
Volume222
Issue number1
DOIs
StatePublished - Sep 2005

Bibliographical note

Funding Information:
Acknowledgements This work was supported by The University Intramural Research Fund Basic Project Awards, by Research Grant Number US-3437–03 from BARD and by the Israel Science Foundation. EP was the recipient of a Forchheimer Visiting Professor Fellowship from the Hebrew University of Jerusalem. We thank Drs. Arie Admon and Tamar Ziv of the Protein Center, Technion, Haifa, Israel for assisting us with the MS analyses, and Gil Shalev for plant culture.

Keywords

  • Flower development
  • Petals
  • Proteome
  • Rose

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