Fluorescence anisotropy to detect in vivo stimulus-induced changes in chemoreceptor packing

Vered Frank; Ady Vaknin

doi:10.1007/978-1-4939-7577-8_20

Fluorescence anisotropy to detect in vivo stimulus-induced changes in chemoreceptor packing

Vered Frank, Ady Vaknin^*

^*Corresponding author for this work

Racah Institute of Physics

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

The anisotropy of the fluorescence emitted from fluorescent proteins, such as yellow fluorescent protein (YFP), is sensitive to Homo-FRET between the proteins. This effect can be used to detect in vivo ligand-induced changes in packing or conformation of tagged chemoreceptors. Such measurements of clustered or dispersed core-signaling units revealed quantitative dose-dependent responses of these sensors.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	247-252
Number of pages	6
DOIs	https://doi.org/10.1007/978-1-4939-7577-8_20
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1729
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC.

Keywords

Chemoreceptors
Fluorescence anisotropy
Homo-FRET

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10.1007/978-1-4939-7577-8_20

Cite this

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Fluorescence anisotropy to detect in vivo stimulus-induced changes in chemoreceptor packing

Abstract

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Keywords

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