Fluorescence anisotropy to detect in vivo stimulus-induced changes in chemoreceptor packing

Vered Frank, Ady Vaknin*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


The anisotropy of the fluorescence emitted from fluorescent proteins, such as yellow fluorescent protein (YFP), is sensitive to Homo-FRET between the proteins. This effect can be used to detect in vivo ligand-induced changes in packing or conformation of tagged chemoreceptors. Such measurements of clustered or dispersed core-signaling units revealed quantitative dose-dependent responses of these sensors.

Original languageAmerican English
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages6
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
The use of fluorescence anisotropy for detecting chemoreceptor responses was pioneered in the lab of Howard C. Berg [5]. This work was funded by Israel Science Foundation.

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC.


  • Chemoreceptors
  • Fluorescence anisotropy
  • Homo-FRET


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