Fluorescence immunoassay based on fluorescer microparticles

Alexander Kamyshny*, Shlomo Magdassi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A novel fluorescence immunoassay based on specific interaction of an antibody with an antigen preadsorbed onto fluorescer (perylene) microparticles (mean diameter 0.8-1.0 μm) is described. The microparticles of perylene are formed by precipitation in IgG solution, and the obtained dispersion is stable in the protein at concentrations higher than 1 mg ml-1. Dissolving the particles in a suitable solvent leads to fluorescence when exciting by light of a proper wavelength. The dependencies of the fluorescence intensity on the concentrations of antigen, antibody and fluorescer were studied. Copyright (C) 2000 Elsevier Science B.V.

Original languageAmerican English
Pages (from-to)13-17
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume18
Issue number1
DOIs
StatePublished - 15 Jun 2000

Keywords

  • Fluorescence
  • IgG
  • Immunoassay
  • Microparticles
  • Perylene

Fingerprint

Dive into the research topics of 'Fluorescence immunoassay based on fluorescer microparticles'. Together they form a unique fingerprint.

Cite this