Abstract
A novel fluorescence immunoassay based on specific interaction of an antibody with an antigen preadsorbed onto fluorescer (perylene) microparticles (mean diameter 0.8-1.0 μm) is described. The microparticles of perylene are formed by precipitation in IgG solution, and the obtained dispersion is stable in the protein at concentrations higher than 1 mg ml-1. Dissolving the particles in a suitable solvent leads to fluorescence when exciting by light of a proper wavelength. The dependencies of the fluorescence intensity on the concentrations of antigen, antibody and fluorescer were studied. Copyright (C) 2000 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 13-17 |
| Number of pages | 5 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 18 |
| Issue number | 1 |
| DOIs | |
| State | Published - 15 Jun 2000 |
Keywords
- Fluorescence
- IgG
- Immunoassay
- Microparticles
- Perylene