Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities

T. D. Ainsworth; M. Fine; L. L. Blackall; O. Hoegh-Guldberg

doi:10.1128/AEM.72.4.3016-3020.2006

Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities

T. D. Ainsworth^*, M. Fine, L. L. Blackall, O. Hoegh-Guldberg

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. This study overcomes these limitations by combining FISH and spectral imaging.

Original language	English
Pages (from-to)	3016-3020
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	72
Issue number	4
DOIs	https://doi.org/10.1128/AEM.72.4.3016-3020.2006
State	Published - Apr 2006
Externally published	Yes

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10.1128/AEM.72.4.3016-3020.2006

Cite this

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AB - Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. This study overcomes these limitations by combining FISH and spectral imaging.

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Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities

Abstract

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