Fluorescent in situ hybridization as an aid to introducing alien genetic variation into wheat

T. E. Miller; S. M. Reader; K. A. Purdie; S. Abbo; R. P. Dunford; I. P. King

doi:10.1007/BF00023956

Fluorescent in situ hybridization as an aid to introducing alien genetic variation into wheat

T. E. Miller^*, S. M. Reader, K. A. Purdie, S. Abbo, R. P. Dunford, I. P. King

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Fluorescent in situ hybridization (FISH) has been used to assess the occurrence and frequency of wheat-alien chromosome pairing in a wheat/Thinopyrum bessarabicum hybrid and in wheat/rye hybrids with different levels of chromosome pairing by examining pollen mother cells at metaphase I of meiosis. The use of FISH to identify the presence and size of alien chromatin in a wheat background is also demonstrated. The value of FISH as an aid to the introgression of alien genetic variation into wheat is discussed.

Original language	English
Pages (from-to)	275-279
Number of pages	5
Journal	Euphytica
Volume	85
Issue number	1-3
DOIs	https://doi.org/10.1007/BF00023956
State	Published - Feb 1995
Externally published	Yes

Keywords

alien introduction
fluorescent in situ hybridization (FISH)
wheat

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10.1007/BF00023956

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title = "Fluorescent in situ hybridization as an aid to introducing alien genetic variation into wheat",

abstract = "Fluorescent in situ hybridization (FISH) has been used to assess the occurrence and frequency of wheat-alien chromosome pairing in a wheat/Thinopyrum bessarabicum hybrid and in wheat/rye hybrids with different levels of chromosome pairing by examining pollen mother cells at metaphase I of meiosis. The use of FISH to identify the presence and size of alien chromatin in a wheat background is also demonstrated. The value of FISH as an aid to the introgression of alien genetic variation into wheat is discussed.",

keywords = "alien introduction, fluorescent in situ hybridization (FISH), wheat",

author = "Miller, {T. E.} and Reader, {S. M.} and Purdie, {K. A.} and S. Abbo and Dunford, {R. P.} and King, {I. P.}",

year = "1995",

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doi = "10.1007/BF00023956",

language = "אנגלית",

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T1 - Fluorescent in situ hybridization as an aid to introducing alien genetic variation into wheat

AU - Miller, T. E.

AU - Reader, S. M.

AU - Purdie, K. A.

AU - Abbo, S.

AU - Dunford, R. P.

AU - King, I. P.

PY - 1995/2

Y1 - 1995/2

N2 - Fluorescent in situ hybridization (FISH) has been used to assess the occurrence and frequency of wheat-alien chromosome pairing in a wheat/Thinopyrum bessarabicum hybrid and in wheat/rye hybrids with different levels of chromosome pairing by examining pollen mother cells at metaphase I of meiosis. The use of FISH to identify the presence and size of alien chromatin in a wheat background is also demonstrated. The value of FISH as an aid to the introgression of alien genetic variation into wheat is discussed.

AB - Fluorescent in situ hybridization (FISH) has been used to assess the occurrence and frequency of wheat-alien chromosome pairing in a wheat/Thinopyrum bessarabicum hybrid and in wheat/rye hybrids with different levels of chromosome pairing by examining pollen mother cells at metaphase I of meiosis. The use of FISH to identify the presence and size of alien chromatin in a wheat background is also demonstrated. The value of FISH as an aid to the introgression of alien genetic variation into wheat is discussed.

KW - alien introduction

KW - fluorescent in situ hybridization (FISH)

KW - wheat

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EP - 279

JO - Euphytica

JF - Euphytica

IS - 1-3

ER -

Fluorescent in situ hybridization as an aid to introducing alien genetic variation into wheat

Abstract

Keywords

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