Abstract
The rupture forces between an aptamer (1)-functionalized AFM tip and a thrombin-modified Au surface are analyzed. The rupture force for a single aptamer/thrombin complex is determined as ̃4.45 pN. The analysis of the system reveals that the rupture forces correspond to the melting of the G-quadruplex structure of the aptamer bound to the thrombin. This melting of the G-quadruplex leads to the dissociation of the aptamer/thrombin complex.
Original language | English |
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Pages (from-to) | 3638-3642 |
Number of pages | 5 |
Journal | Analytical Chemistry |
Volume | 78 |
Issue number | 11 |
DOIs | |
State | Published - 1 Jun 2006 |