Following the biocatalytic activities of glucose oxidase by electrochemically cross-linked enzyme-Pt nanoparticles composite electrodes

Lily Bahshi; Marco Frasconi; Ran Tel-Vered; Omer Yehezkeli; Itamar Willner

doi:10.1021/ac801398m

Following the biocatalytic activities of glucose oxidase by electrochemically cross-linked enzyme-Pt nanoparticles composite electrodes

Lily Bahshi, Marco Frasconi, Ran Tel-Vered, Omer Yehezkeli, Itamar Willner^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

An integrated platinum nanoparticles (NPs)/glucose oxidase (GOx) composite film associated with a Au electrode is used to follow the biocatalytic activities of the enzyme. The film is assembled on a Au electrode by the electropolymerization of thioaniline-functionalized Pt NPs and thioaniline-modified GOx. The resulting enzyme/Pt NPs-functionalized electrode stimulates the O₂ oxidation of glucose to gluconic acid and H ₂O₂. The modified electrode is then implemented to follow the activity of the enzyme by the electrochemical monitoring of the generated H₂O₂. The effect of the composition of the Pt NPs/GOx cross-linked nanostructures and the optimal conditions for the preparation of the electrodes are discussed.

Original language	English
Pages (from-to)	8253-8259
Number of pages	7
Journal	Analytical Chemistry
Volume	80
Issue number	21
DOIs	https://doi.org/10.1021/ac801398m
State	Published - 1 Nov 2008

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abstract = "An integrated platinum nanoparticles (NPs)/glucose oxidase (GOx) composite film associated with a Au electrode is used to follow the biocatalytic activities of the enzyme. The film is assembled on a Au electrode by the electropolymerization of thioaniline-functionalized Pt NPs and thioaniline-modified GOx. The resulting enzyme/Pt NPs-functionalized electrode stimulates the O2 oxidation of glucose to gluconic acid and H 2O2. The modified electrode is then implemented to follow the activity of the enzyme by the electrochemical monitoring of the generated H2O2. The effect of the composition of the Pt NPs/GOx cross-linked nanostructures and the optimal conditions for the preparation of the electrodes are discussed.",

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AU - Yehezkeli, Omer

AU - Willner, Itamar

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Following the biocatalytic activities of glucose oxidase by electrochemically cross-linked enzyme-Pt nanoparticles composite electrodes

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