FoXS, FoXSDock and MultiFoXS: Single-state and multi-state structural modeling of proteins and their complexes based on SAXS profiles

Dina Schneidman-Duhovny; Michal Hammel; John A. Tainer; Andrej Sali

doi:10.1093/nar/gkw389

FoXS, FoXSDock and MultiFoXS: Single-state and multi-state structural modeling of proteins and their complexes based on SAXS profiles

Dina Schneidman-Duhovny^*, Michal Hammel, John A. Tainer, Andrej Sali^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

374 Scopus citations

Abstract

Small Angle X-ray Scattering (SAXS) is an increasingly common and useful technique for structural characterization of molecules in solution. A SAXS experiment determines the scattering intensity of a molecule as a function of spatial frequency, termed SAXS profile. Here, we describe three web servers for modeling atomic structures based on SAXS profiles. FoXS (Fast X-Ray Scattering) rapidly computes a SAXS profile of a given atomistic model and fits it to an experimental profile. FoXSDock docks two rigid protein structures based on a SAXS profile of their complex. MultiFoXS computes a population-weighted ensemble starting from a single input structure by fitting to a SAXS profile of the protein in solution. We describe the interfaces and capabilities of the servers (salilab.org/foxs), followed by demonstrating their application on Interleukin-33 (IL-33) and its primary receptor ST2.

Original language	English
Pages (from-to)	W424-W429
Journal	Nucleic Acids Research
Volume	44
Issue number	1
DOIs	https://doi.org/10.1093/nar/gkw389
State	Published - 2016
Externally published	Yes

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© The Author(s) 2016.

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FoXS, FoXSDock and MultiFoXS: Single-state and multi-state structural modeling of proteins and their complexes based on SAXS profiles

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