Fraction of Cholesterol Undergoing Spontaneous Exchange between Small Unilamellar Phosphatidylcholine Vesicles

Liliana K. Bar, Y. Barenholz, T. E. Thompson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

The kinetics of the spontaneous exchange of [3H]cholesterol between small unilamellar vesicles of phosphatidylcholine has been reexamined. Although first-order exchange kinetics were observed (k = 0.0117 min-1), in good agreement with previous studies, about 20% of the total cholesterol was found to be nonexchangeable in the 8-h time frame of the experiments. The size of this nonexchangeable pool was found to depend on the type of phospholipid and the temperature. It seems probable that the two pools of cholesterol defined in these experiments reflect the complex phase structure of the cholesterol-phosphatidylcholine vesicles.

Original languageEnglish
Pages (from-to)6701-6705
Number of pages5
JournalBiochemistry
Volume25
Issue number21
DOIs
StatePublished - Oct 1986

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