TY - JOUR
T1 - Fractionation of theiler's virus-infected BHK21 cell homogenates
T2 - Isolation of virus-induced membranes
AU - Frankel, Gad
AU - Lorch, Yahu
AU - Karlik, Paula
AU - Friedmann, Adam
PY - 1987/6
Y1 - 1987/6
N2 - A purified fraction containing unique membranes entrapping virions was isolated from homogenates of cells infected with the DA strain of Theiler's virus, after high-speed centrifugation through a sucrose gradient. This fraction, sedimented at 45-50% sucrose, was only found in cells infected with the DA strain but not in cells infected with the GDVII strain of Theiler's virus or in mock-infected cells. Immunogold staining of the membranes entrapping virions, using antivirus IgG antibodies, revealed that the membranes entrapping virions did not incorporate viral capsid antigens.
AB - A purified fraction containing unique membranes entrapping virions was isolated from homogenates of cells infected with the DA strain of Theiler's virus, after high-speed centrifugation through a sucrose gradient. This fraction, sedimented at 45-50% sucrose, was only found in cells infected with the DA strain but not in cells infected with the GDVII strain of Theiler's virus or in mock-infected cells. Immunogold staining of the membranes entrapping virions, using antivirus IgG antibodies, revealed that the membranes entrapping virions did not incorporate viral capsid antigens.
UR - http://www.scopus.com/inward/record.url?scp=0023188044&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(87)90220-0
DO - 10.1016/0042-6822(87)90220-0
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C2 - 3035792
AN - SCOPUS:0023188044
SN - 0042-6822
VL - 158
SP - 452
EP - 455
JO - Virology
JF - Virology
IS - 2
ER -