Abstract
A method is presented for reliable use of pooled chicken blood samples for estimation of microsatellite frequencies by direct polymerase chain reaction (PCR) amplification of DNA. This method overcomes the variability of hematocrit values in individual chickens and eliminates the step of DNA preparation. The estimated frequencies of polymorphic alleles in fresh and frozen pooled blood samples were similar to those obtained by calculating these frequencies from the individual genotyping. When frozen pooled blood samples are used, pools should be prepared prior to their freezing.
| Original language | English |
|---|---|
| Pages (from-to) | 902-904 |
| Number of pages | 3 |
| Journal | Poultry Science |
| Volume | 77 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1998 |
Keywords
- Chicken
- Genotyping
- Microsatellite
- Polymerase chain reaction
- Pooled blood