From Agrobacterium to viral vectors: Genome modification of plant cells by rare cutting restriction enzymes

Ira Marton, Arik Honig, Ayelet Omid, Noam De Costa, Elena Marhevka, Barry Cohen, Amir Zuker, Alexander Vainstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Researchers and biotechnologists require methods to accurately modify the genome of higher eukaryotic cells. Such modifications include, but are not limited to, site-specific mutagenesis, site-specific insertion of foreign DNA, and replacement and deletion of native sequences. Accurate genome modifications in plant species have been rather limited, with only a handful of plant species and genes being modified through the use of early genome-editing techniques. The development of rare-cutting restriction enzymes as a tool for the induction of site-specific genomic double-strand breaks and their introduction as a reliable tool for genome modification in animals, animal cells and human cell lines have paved the way for the adaptation of rare-cutting restriction enzymes to genome editing in plant cells. Indeed, the number of plant species and genes which have been successfully edited using zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and engineered homing endonucleases is on the rise. In our review, we discuss the basics of rare-cutting restriction enzyme-mediated genome-editing technology with an emphasis on its application in plant species.

Original languageAmerican English
Pages (from-to)639-650
Number of pages12
JournalInternational Journal of Developmental Biology
Issue number6-8
StatePublished - 2013


  • Genome editing
  • Homing endonucleases
  • TALENs
  • Viral vectors
  • ZFNs


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