Fruiting body formation by Bacillus subtilis

Steven S. Branda, José Eduardo González-Pastor, Sigal Ben-Yehuda, Richard Losick, Roberto Kolter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

885 Scopus citations

Abstract

Spore formation by the bacterium Bacillus subtilis has long been studied as a model for cellular differentiation, but predominantly as a single cell. When analyzed within the context of highly structured, surface-associated communities (biofilms), spore formation was discovered to have heretofore unsuspected spatial organization. Initially, motile cells differentiated into aligned chains of attached cells that eventually produced aerial structures, or fruiting bodies, that served as preferential sites for sporulation. Fruiting body formation depended on regulatory genes required early in sporulation and on genes evidently needed for exopolysaccharide and surfactin production. The formation of aerial structures was robust in natural isolates but not in laboratory strains, an indication that multicellularity has been lost during domestication of B. subtilis. Other microbial differentiation processes long thought to involve only single cells could display the spatial organization characteristic of multicellular organisms when studied with recent natural isolates.

Original languageAmerican English
Pages (from-to)11621-11626
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number20
DOIs
StatePublished - 25 Sep 2001
Externally publishedYes

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