Functional Assays Evaluating Immunosuppression Mediated by Myeloid-Derived Suppressor Cells

Or Reuven, Ivan Mikula, Hadas Ashkenazi-Preiser, Nira Twaik, Kerem Ben-Meir, Yaron Meirow, Leonor Daniel, Guy Kariv, Mahdi Kurd, Michal Baniyash*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Myeloid-derived suppressor cells (MDSCs) are heterogenous populations of immature myeloid cells that can be divided into two main subpopulations, polymorphonuclear (PMN) MDSCs and monocytic (M) MDSCs. These cells accumulate during chronic inflammation, characterizing an array of pathologies such as cancer, inflammatory bowel disease, and infectious and autoimmune diseases, and induce immunosuppression. The suppressive effects of MDSCs on the immune system are studied mainly when focusing on their features, functions, and impact on target cells such as T cells, natural killer cells, and B cells, among others. Herein, we describe methods for the analysis of MDSC immunosuppressive features and functions, measuring different mediators that contribute to their activities and how they impact on T cell function. The protocols described are a continuation to those in a companion Current Protocols article by Reuven et al. (2022), which uses a generated single-cell suspension and isolated cells to test their activity.

Original languageAmerican English
Article numbere557
JournalCurrent Protocols
Issue number10
StatePublished - Oct 2022

Bibliographical note

Funding Information:
The authors gratefully acknowledge the support of the Society of Research Associates of the Lautenberg Center and the Harold B. Abramson Chair in Immunology. The authors also thank the grant support from the Israel Science Foundation, the Israeli Ministry of Health, the Israel Cancer Research Fund, The Israel Ministry of Science and Technology, the Gross Foundation, the Bruce and Baila Waldholtz Funds, and the Joseph and Matilda Melnick Funds.

Publisher Copyright:
© 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.


  • MDSC
  • cell culture
  • chronic inflammation
  • flow cytometry
  • immunosuppression
  • inflammatory mouse model
  • myeloid-derived suppressor cell


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