Functional deficits resulting from laser-induced damage in the rat retina

Gil Ben-Shlomo; Mark Belokopytov; Mordechai Rosner; Galina Dubinsky; Michael Belkin; Yoram Epstein; Ron Ofri

doi:10.1002/lsm.20351

Functional deficits resulting from laser-induced damage in the rat retina

Gil Ben-Shlomo, Mark Belokopytov, Mordechai Rosner, Galina Dubinsky, Michael Belkin^*, Yoram Epstein, Ron Ofri

^*Corresponding author for this work

The Koret School of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Background and Objectives: To determine the threshold for electrophysiological detection of functional changes after laser photocoagulation in rats, and to correlate the functional damage with retinal morphology. Study Design/Materials and Methods: Argon-laser lesions, covering a quarter or half of the retina, were produced in the right eyes of 25 rats. Eyes were evaluated by flash electroretinography (ERG) and histologically at 3, 21, and 60 days after lasering. Results: Lasering of half the retina, but not of a quarter, produced a significant decrease in signals at all time points. Some functional recovery was evident 60 days after injury, and was consistent with concurrent morphological healing. Conclusions: In rats, lasering of half the retina, but not a quarter, leads to significant deficits in outer retinal functions over a 2-month period. Thereafter some recovery occurs, presumably as a result of migration of photoreceptors from undamaged areas and their retinal remodeling.

Original language	American English
Pages (from-to)	689-694
Number of pages	6
Journal	Lasers in Surgery and Medicine
Volume	38
Issue number	7
DOIs	https://doi.org/10.1002/lsm.20351
State	Published - Aug 2006

Keywords

Electroretinogram
Laser
Retinal injury
Wound healing

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10.1002/lsm.20351

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title = "Functional deficits resulting from laser-induced damage in the rat retina",

abstract = "Background and Objectives: To determine the threshold for electrophysiological detection of functional changes after laser photocoagulation in rats, and to correlate the functional damage with retinal morphology. Study Design/Materials and Methods: Argon-laser lesions, covering a quarter or half of the retina, were produced in the right eyes of 25 rats. Eyes were evaluated by flash electroretinography (ERG) and histologically at 3, 21, and 60 days after lasering. Results: Lasering of half the retina, but not of a quarter, produced a significant decrease in signals at all time points. Some functional recovery was evident 60 days after injury, and was consistent with concurrent morphological healing. Conclusions: In rats, lasering of half the retina, but not a quarter, leads to significant deficits in outer retinal functions over a 2-month period. Thereafter some recovery occurs, presumably as a result of migration of photoreceptors from undamaged areas and their retinal remodeling.",

keywords = "Electroretinogram, Laser, Retinal injury, Wound healing",

author = "Gil Ben-Shlomo and Mark Belokopytov and Mordechai Rosner and Galina Dubinsky and Michael Belkin and Yoram Epstein and Ron Ofri",

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T1 - Functional deficits resulting from laser-induced damage in the rat retina

AU - Ben-Shlomo, Gil

AU - Belokopytov, Mark

AU - Rosner, Mordechai

AU - Dubinsky, Galina

AU - Belkin, Michael

AU - Epstein, Yoram

AU - Ofri, Ron

PY - 2006/8

Y1 - 2006/8

N2 - Background and Objectives: To determine the threshold for electrophysiological detection of functional changes after laser photocoagulation in rats, and to correlate the functional damage with retinal morphology. Study Design/Materials and Methods: Argon-laser lesions, covering a quarter or half of the retina, were produced in the right eyes of 25 rats. Eyes were evaluated by flash electroretinography (ERG) and histologically at 3, 21, and 60 days after lasering. Results: Lasering of half the retina, but not of a quarter, produced a significant decrease in signals at all time points. Some functional recovery was evident 60 days after injury, and was consistent with concurrent morphological healing. Conclusions: In rats, lasering of half the retina, but not a quarter, leads to significant deficits in outer retinal functions over a 2-month period. Thereafter some recovery occurs, presumably as a result of migration of photoreceptors from undamaged areas and their retinal remodeling.

AB - Background and Objectives: To determine the threshold for electrophysiological detection of functional changes after laser photocoagulation in rats, and to correlate the functional damage with retinal morphology. Study Design/Materials and Methods: Argon-laser lesions, covering a quarter or half of the retina, were produced in the right eyes of 25 rats. Eyes were evaluated by flash electroretinography (ERG) and histologically at 3, 21, and 60 days after lasering. Results: Lasering of half the retina, but not of a quarter, produced a significant decrease in signals at all time points. Some functional recovery was evident 60 days after injury, and was consistent with concurrent morphological healing. Conclusions: In rats, lasering of half the retina, but not a quarter, leads to significant deficits in outer retinal functions over a 2-month period. Thereafter some recovery occurs, presumably as a result of migration of photoreceptors from undamaged areas and their retinal remodeling.

KW - Electroretinogram

KW - Laser

KW - Retinal injury

KW - Wound healing

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Functional deficits resulting from laser-induced damage in the rat retina

Abstract

Keywords

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