Functional mimicry of a protein hormone by a peptide agonist: The EPO receptor complex at 2.8 Å

Oded Livnah, Enrico A. Stura, Dana L. Johnson, Steven A. Middleton, Linda S. Mulcahy, Nicholas C. Wrighton, William J. Dower, Linda K. Jolliffe, Ian A. Wilson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

566 Scopus citations

Abstract

The functional mimicry of a protein by an unrelated small molecule has been a formidable challenge. Now, however, the biological activity of a 166- residue hematopoietic growth hormone, erythropoietin (EPO), with its class 1 cytokine receptor has been mimicked by a 20-residue cyclic peptide unrelated in sequence to the natural ligand. The crystal structure at 2.8 Å resolution of a complex of this agonist peptide with the extracellular domain of EPO receptor reveals that a peptide dimer induces an almost perfect twofold dimerization of the receptor. The dimer assembly differs from that of the human growth hormone (hGH) receptor complex and suggests that more than one mode of dimerization may be able to induce signal transduction and cell proliferation. The EPO receptor binding site, defined by peptide interaction, corresponds to the smaller functional epitope identified for hGH receptor. Similarly, the EPO mimetic peptide ligand can be considered as a minimal hormone, and suggests the design of nonpeptidic smell molecule mimetics for EPO and other cytokines may indeed be achievable.

Original languageAmerican English
Pages (from-to)464-471
Number of pages8
JournalScience
Volume273
Issue number5274
DOIs
StatePublished - 26 Jul 1996
Externally publishedYes

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