Functions of Opsins in Drosophila Taste

Nicole Y. Leung, Dhananjay P. Thakur, Adishthi S. Gurav, Sang Hoon Kim, Antonella Di Pizio, Masha Y. Niv, Craig Montell*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Rhodopsin is a light receptor comprised of an opsin protein and a light-sensitive retinal chromophore. Despite more than a century of scrutiny, there is no evidence that opsins function in chemosensation. Here, we demonstrate that three Drosophila opsins, Rh1, Rh4, and Rh7, are needed in gustatory receptor neurons to sense a plant-derived bitter compound, aristolochic acid (ARI). The gustatory requirements for these opsins are light-independent and do not require retinal. The opsins enabled flies to detect lower concentrations of aristolochic acid by initiating an amplification cascade that includes a G-protein, phospholipase Cβ, and the TRP channel, TRPA1. In contrast, responses to higher levels of the bitter compound were mediated through direct activation of TRPA1. Our study reveals roles for opsins in chemosensation and raise questions concerning the original roles for these classical G-protein-coupled receptors.

Original languageAmerican English
Pages (from-to)1367-1379.e6
JournalCurrent Biology
Volume30
Issue number8
DOIs
StatePublished - 20 Apr 2020

Bibliographical note

Publisher Copyright:
© 2020 The Author(s)

Keywords

  • Drosophila
  • GPCR
  • TRP channel
  • chromophore
  • gustatory
  • labellum
  • opsin
  • phospholipase C
  • retinal
  • rhodopsin

Fingerprint

Dive into the research topics of 'Functions of Opsins in Drosophila Taste'. Together they form a unique fingerprint.

Cite this