Fluorescence-based techniques enable researchers to monitor physiologic processes, specifically fungal cell viability and death, during cellular encounters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells to interrogate genetic, pharmacologic, and cellular determinants that shape host–fungal cell outcomes. This chapter reviews the techniques and applications of fluorescent fungal reporters of viability, with emphasis on the North American endemic dimorphic fungus, Blastomyces dermatitidis.
|Original language||American English|
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||9|
|State||Published - 2021|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
The work is supported by NIH grant RO1 AI093808 (to T.M.H.), P30 CA008748 (to MSKCC), and a Burroughs Wellcome Fund Investigator in the Pathogenesis of Infectious Disease Award (to T. M.H.) and by NIH R01 AI035681 (to B.S.K.) and American Heart Association Postdoctoral Fellowship 17POST32790004 (to J.S.F.).
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.
- Fluorescent reporters
- Host-Blastomyces interactions