Fungal Bioreporters to Monitor Outcomes of Blastomyces: Host–Cell Interactions

Jeffrey Scott Fites; Neta Shlezinger; Tobias M. Hohl; Bruce S. Klein

doi:10.1007/978-1-0716-1182-1_7

Fungal Bioreporters to Monitor Outcomes of Blastomyces: Host–Cell Interactions

Jeffrey Scott Fites, Neta Shlezinger, Tobias M. Hohl^*, Bruce S. Klein

^*Corresponding author for this work

The Koret School of Veterinary Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Fluorescence-based techniques enable researchers to monitor physiologic processes, specifically fungal cell viability and death, during cellular encounters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells to interrogate genetic, pharmacologic, and cellular determinants that shape host–fungal cell outcomes. This chapter reviews the techniques and applications of fluorescent fungal reporters of viability, with emphasis on the North American endemic dimorphic fungus, Blastomyces dermatitidis.

Original language	American English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	111-119
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-0716-1182-1_7
State	Published - 2021

Publication series

Name	Methods in Molecular Biology
Volume	2260
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
The work is supported by NIH grant RO1 AI093808 (to T.M.H.), P30 CA008748 (to MSKCC), and a Burroughs Wellcome Fund Investigator in the Pathogenesis of Infectious Disease Award (to T. M.H.) and by NIH R01 AI035681 (to B.S.K.) and American Heart Association Postdoctoral Fellowship 17POST32790004 (to J.S.F.).

Publisher Copyright:
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Fluorescent reporters
Host-Blastomyces interactions

Access to Document

10.1007/978-1-0716-1182-1_7

Cite this

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abstract = "Fluorescence-based techniques enable researchers to monitor physiologic processes, specifically fungal cell viability and death, during cellular encounters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells to interrogate genetic, pharmacologic, and cellular determinants that shape host–fungal cell outcomes. This chapter reviews the techniques and applications of fluorescent fungal reporters of viability, with emphasis on the North American endemic dimorphic fungus, Blastomyces dermatitidis.",

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N1 - Funding Information: The work is supported by NIH grant RO1 AI093808 (to T.M.H.), P30 CA008748 (to MSKCC), and a Burroughs Wellcome Fund Investigator in the Pathogenesis of Infectious Disease Award (to T. M.H.) and by NIH R01 AI035681 (to B.S.K.) and American Heart Association Postdoctoral Fellowship 17POST32790004 (to J.S.F.). Publisher Copyright: © 2021, Springer Science+Business Media, LLC, part of Springer Nature.

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AB - Fluorescence-based techniques enable researchers to monitor physiologic processes, specifically fungal cell viability and death, during cellular encounters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells to interrogate genetic, pharmacologic, and cellular determinants that shape host–fungal cell outcomes. This chapter reviews the techniques and applications of fluorescent fungal reporters of viability, with emphasis on the North American endemic dimorphic fungus, Blastomyces dermatitidis.

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Fungal Bioreporters to Monitor Outcomes of Blastomyces: Host–Cell Interactions

Abstract

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Keywords

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