Further characterization of the ataxia-telangiectasia clastogenic factor by reversed-phase liquid chromatography

Meira Shaham; Michael Chorev; Yechiel Becker

doi:10.1016/0168-1656(84)90026-9

Further characterization of the ataxia-telangiectasia clastogenic factor by reversed-phase liquid chromatography

Meira Shaham^*
, Michael Chorev
, Yechiel Becker

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The clastogenic factor present in medium conditioned by ataxia-telangiectasia (A-T) fibroblast cultures was chromatographed on LiChrosorb RP-8 columns and was eluted with a solution of 20% methanol in 0.005 M NH₄HCO₃. Based on this property, the A-T clastogenic factor was isolated from a C₈ column by high-performance liquid chromatography (HPLC). A specific fraction of the HPLC eluate contained the clastogenic factor. This method makes possible the purification of the A-T clastogenic factor for further analysis.

Original language	English
Pages (from-to)	351-354
Number of pages	4
Journal	Journal of Biotechnology
Volume	1
Issue number	5-6
DOIs	https://doi.org/10.1016/0168-1656(84)90026-9
State	Published - Dec 1984
Externally published	Yes

Keywords

ataxia-telangiectasia
chromosome damage
clastogenic factor
reversed-phase liquid chromatography

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10.1016/0168-1656(84)90026-9

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title = "Further characterization of the ataxia-telangiectasia clastogenic factor by reversed-phase liquid chromatography",

abstract = "The clastogenic factor present in medium conditioned by ataxia-telangiectasia (A-T) fibroblast cultures was chromatographed on LiChrosorb RP-8 columns and was eluted with a solution of 20\% methanol in 0.005 M NH4HCO3. Based on this property, the A-T clastogenic factor was isolated from a C8 column by high-performance liquid chromatography (HPLC). A specific fraction of the HPLC eluate contained the clastogenic factor. This method makes possible the purification of the A-T clastogenic factor for further analysis.",

keywords = "ataxia-telangiectasia, chromosome damage, clastogenic factor, reversed-phase liquid chromatography",

author = "Meira Shaham and Michael Chorev and Yechiel Becker",

year = "1984",

month = dec,

doi = "10.1016/0168-1656(84)90026-9",

language = "אנגלית",

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journal = "Journal of Biotechnology",

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T1 - Further characterization of the ataxia-telangiectasia clastogenic factor by reversed-phase liquid chromatography

AU - Shaham, Meira

AU - Chorev, Michael

AU - Becker, Yechiel

PY - 1984/12

Y1 - 1984/12

N2 - The clastogenic factor present in medium conditioned by ataxia-telangiectasia (A-T) fibroblast cultures was chromatographed on LiChrosorb RP-8 columns and was eluted with a solution of 20% methanol in 0.005 M NH4HCO3. Based on this property, the A-T clastogenic factor was isolated from a C8 column by high-performance liquid chromatography (HPLC). A specific fraction of the HPLC eluate contained the clastogenic factor. This method makes possible the purification of the A-T clastogenic factor for further analysis.

AB - The clastogenic factor present in medium conditioned by ataxia-telangiectasia (A-T) fibroblast cultures was chromatographed on LiChrosorb RP-8 columns and was eluted with a solution of 20% methanol in 0.005 M NH4HCO3. Based on this property, the A-T clastogenic factor was isolated from a C8 column by high-performance liquid chromatography (HPLC). A specific fraction of the HPLC eluate contained the clastogenic factor. This method makes possible the purification of the A-T clastogenic factor for further analysis.

KW - ataxia-telangiectasia

KW - chromosome damage

KW - clastogenic factor

KW - reversed-phase liquid chromatography

UR - https://www.scopus.com/pages/publications/0021706910

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AN - SCOPUS:0021706910

SN - 0168-1656

VL - 1

SP - 351

EP - 354

JO - Journal of Biotechnology

JF - Journal of Biotechnology

IS - 5-6

ER -

Further characterization of the ataxia-telangiectasia clastogenic factor by reversed-phase liquid chromatography

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Keywords

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