Abstract
The separation of 2′-5′ dinucleoside monophosphates from their corresponding 3′-5′ isomers on QAE-Sephadex A-25 (bicarbonate form) is described. The column is loaded with a mixture of the two isomers and eluted with a linear gradient of ammonium bicarbonate (from 0.02 to 0.2 M) The two isomers appear in two different peaks, and the nucleotidic material is isolated by lyophilization. In addition, the separation of 2′-5′ dinucleoside monophosphates from the corresponding 3′-5′ isomers by paper chromatography and by thin-layer chromatography on aluminium cards coated with cellulose powder is described.
Original language | English |
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Pages (from-to) | 139-146 |
Number of pages | 8 |
Journal | Journal of Chromatography A |
Volume | 79 |
Issue number | C |
DOIs | |
State | Published - 16 May 1973 |