Further studies on the chromatographic behaviour of dinucleoside monophosphates

I. Barzilay*, J. L. Sussman, Y. Lapidot

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The separation of 2′-5′ dinucleoside monophosphates from their corresponding 3′-5′ isomers on QAE-Sephadex A-25 (bicarbonate form) is described. The column is loaded with a mixture of the two isomers and eluted with a linear gradient of ammonium bicarbonate (from 0.02 to 0.2 M) The two isomers appear in two different peaks, and the nucleotidic material is isolated by lyophilization. In addition, the separation of 2′-5′ dinucleoside monophosphates from the corresponding 3′-5′ isomers by paper chromatography and by thin-layer chromatography on aluminium cards coated with cellulose powder is described.

Original languageEnglish
Pages (from-to)139-146
Number of pages8
JournalJournal of Chromatography A
Volume79
Issue numberC
DOIs
StatePublished - 16 May 1973

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