Fusion-mediated implantation of band 3 into living cells. A new system to study degradation of membrane proteins

Michel Beigel*, Abraham Loyter

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Sendai virus (SV) glycoproteins were co-reconstituted with the erythrocyte membrane polypeptide designated as band 3 (B3). Fusion of the hybrid vesicles, SV-B3, with L. HeLa and Friend erythroleukemic (FEL) cells led to fusion-mediated implantation of the erythrocyte band 3 into the plasma membranes of the above cells. By the use of radiolabelled band 3 ([3H]H2DIDS-B3) it was possible to demonstrate that about 1-4 × 106 molecules of band 3 can be transferred to the membranes of each of the above cell lines. Cultivation of the recipient cells in growth medium resulted in gradual decrease of the cell-associated counts. The reduction pattern followed first-order kinetics, reaching t 1 2 of disappearance at about 25-30 h in the case of L and HeLa cells, and 57 h in the case of FEL cells. NH4Cl added to the growth medium significantly inhibited the disappearance of band 3 during growth. The studies of the present work indicate that the reduction in the cell-associated band 3 during growth may represent degradation of this polypeptide by the recipient cells.

Original languageEnglish
Pages (from-to)95-103
Number of pages9
JournalExperimental Cell Research
Volume148
Issue number1
DOIs
StatePublished - 1 Oct 1983

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