Fusion of chicken erythrocytes by phospholipase C (Clostridium perfringens). The requirement for hemolytic and hydrolytic factors for fusion

Esther Sabban; Abraham Loyter

doi:10.1016/0304-4165(74)90031-2

Fusion of chicken erythrocytes by phospholipase C (Clostridium perfringens). The requirement for hemolytic and hydrolytic factors for fusion

Esther Sabban^*, Abraham Loyter

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Phospholipase C (Clostridium perfringens) preparations are able to induce fusion of chicken erythrocytes only in the presence of low concentrations (0.6 mM) of Mn²⁺. Anti-phospholipase C serum inhibits hemolysis and fusion of the cells only when added during the first 20 min of the incubation time. Heated phospholipase C preparations which fail to hemolyze chicken erythrocytes are able to induce fusion only in the presence of another hemolytic reagent such as prymnesin (an hemolytic toxin from Prymnesium parvum).

Original language	English
Pages (from-to)	100-109
Number of pages	10
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	362
Issue number	1
DOIs	https://doi.org/10.1016/0304-4165(74)90031-2
State	Published - 7 Aug 1974

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abstract = "Phospholipase C (Clostridium perfringens) preparations are able to induce fusion of chicken erythrocytes only in the presence of low concentrations (0.6 mM) of Mn2+. Anti-phospholipase C serum inhibits hemolysis and fusion of the cells only when added during the first 20 min of the incubation time. Heated phospholipase C preparations which fail to hemolyze chicken erythrocytes are able to induce fusion only in the presence of another hemolytic reagent such as prymnesin (an hemolytic toxin from Prymnesium parvum).",

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AU - Loyter, Abraham

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N2 - Phospholipase C (Clostridium perfringens) preparations are able to induce fusion of chicken erythrocytes only in the presence of low concentrations (0.6 mM) of Mn2+. Anti-phospholipase C serum inhibits hemolysis and fusion of the cells only when added during the first 20 min of the incubation time. Heated phospholipase C preparations which fail to hemolyze chicken erythrocytes are able to induce fusion only in the presence of another hemolytic reagent such as prymnesin (an hemolytic toxin from Prymnesium parvum).

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Fusion of chicken erythrocytes by phospholipase C (Clostridium perfringens). The requirement for hemolytic and hydrolytic factors for fusion

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