TY - JOUR
T1 - Fusion of enveloped viruses with sperm cells
T2 - Interaction of sendai, influenza, and semliki forest viruses with bull spermatozoa
AU - Nussbaum, Ofer
AU - Laster, Judith
AU - Loyter, Abraham
PY - 1993/5
Y1 - 1993/5
N2 - Incubation of fluorescently labeled Sendai, influenza, as well as Semliki Forest viruses with bull sperm cells resulted in fluorescence dequenching. Fluorescence dequenching was observed with Sendai virus at pH 7.4 while with influenza and Semliki Forest viruses at pH 5.0, a pH value which is required for triggering their fusogenic activity. Control experiments performed with nonfusogenic Sendai and influenza viruses, or with bull sperm cells from which the viral receptors have been removed by treatment with neuraminidase, showed little fluorescence dequenching. These results clearly indicate that animal enveloped viruses are able to interact and to fuse with bull sperm cells. The possibility that following virus-sperm fusion spermatozoa can serve as a carrier of the virus genome and introduce it into recipient eggs during fertilization is discussed.
AB - Incubation of fluorescently labeled Sendai, influenza, as well as Semliki Forest viruses with bull sperm cells resulted in fluorescence dequenching. Fluorescence dequenching was observed with Sendai virus at pH 7.4 while with influenza and Semliki Forest viruses at pH 5.0, a pH value which is required for triggering their fusogenic activity. Control experiments performed with nonfusogenic Sendai and influenza viruses, or with bull sperm cells from which the viral receptors have been removed by treatment with neuraminidase, showed little fluorescence dequenching. These results clearly indicate that animal enveloped viruses are able to interact and to fuse with bull sperm cells. The possibility that following virus-sperm fusion spermatozoa can serve as a carrier of the virus genome and introduce it into recipient eggs during fertilization is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0027193099&partnerID=8YFLogxK
U2 - 10.1006/excr.1993.1114
DO - 10.1006/excr.1993.1114
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AN - SCOPUS:0027193099
SN - 0014-4827
VL - 206
SP - 11
EP - 15
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -