Fusion of germinating watermelon pollen tubes with liposomes

Alexander E. Gad; Ben zion Zeewi; Arie Altman

doi:10.1016/0168-9452(88)90043-X

Fusion of germinating watermelon pollen tubes with liposomes

Alexander E. Gad, Ben zion Zeewi, Arie Altman^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Optimal conditions for germination of watermelon (Citrullus vulgaris, var. 'Sugar Baby') pollen were established and the effect of large unilamellar vesicles (LUV) and several fusing agents was studied. Both polylysine and poly-(ethylene glycol) (PEG) inhibited pollen germination, but polylysine was less harmful than PEG, when applied in combination with LUV. Binding of LUV to the pollen tubes was visualized in scanning electron micrographs. The binding took place only in the presence of polylysine. Incubation of LUV with germinating pollen in the presence of polylysine resulted in their fusion with pollen membranes as evidenced by a resonance energy transfer assay. Addition of CaCl₂ promoted the fusion even further. The data are discussed with respect to the possibility of transformation of pollen by liposome-mediated introduction of nucleic acids into the pollen tube.

Original language	English
Pages (from-to)	69-75
Number of pages	7
Journal	Plant Science
Volume	55
Issue number	1
DOIs	https://doi.org/10.1016/0168-9452(88)90043-X
State	Published - 1988

Keywords

Citrullus vulgaris
fusion
liposomes
pollen germination
polylysine

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10.1016/0168-9452(88)90043-X

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title = "Fusion of germinating watermelon pollen tubes with liposomes",

abstract = "Optimal conditions for germination of watermelon (Citrullus vulgaris, var. 'Sugar Baby') pollen were established and the effect of large unilamellar vesicles (LUV) and several fusing agents was studied. Both polylysine and poly-(ethylene glycol) (PEG) inhibited pollen germination, but polylysine was less harmful than PEG, when applied in combination with LUV. Binding of LUV to the pollen tubes was visualized in scanning electron micrographs. The binding took place only in the presence of polylysine. Incubation of LUV with germinating pollen in the presence of polylysine resulted in their fusion with pollen membranes as evidenced by a resonance energy transfer assay. Addition of CaCl2 promoted the fusion even further. The data are discussed with respect to the possibility of transformation of pollen by liposome-mediated introduction of nucleic acids into the pollen tube.",

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AU - Gad, Alexander E.

AU - Zeewi, Ben zion

AU - Altman, Arie

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N2 - Optimal conditions for germination of watermelon (Citrullus vulgaris, var. 'Sugar Baby') pollen were established and the effect of large unilamellar vesicles (LUV) and several fusing agents was studied. Both polylysine and poly-(ethylene glycol) (PEG) inhibited pollen germination, but polylysine was less harmful than PEG, when applied in combination with LUV. Binding of LUV to the pollen tubes was visualized in scanning electron micrographs. The binding took place only in the presence of polylysine. Incubation of LUV with germinating pollen in the presence of polylysine resulted in their fusion with pollen membranes as evidenced by a resonance energy transfer assay. Addition of CaCl2 promoted the fusion even further. The data are discussed with respect to the possibility of transformation of pollen by liposome-mediated introduction of nucleic acids into the pollen tube.

AB - Optimal conditions for germination of watermelon (Citrullus vulgaris, var. 'Sugar Baby') pollen were established and the effect of large unilamellar vesicles (LUV) and several fusing agents was studied. Both polylysine and poly-(ethylene glycol) (PEG) inhibited pollen germination, but polylysine was less harmful than PEG, when applied in combination with LUV. Binding of LUV to the pollen tubes was visualized in scanning electron micrographs. The binding took place only in the presence of polylysine. Incubation of LUV with germinating pollen in the presence of polylysine resulted in their fusion with pollen membranes as evidenced by a resonance energy transfer assay. Addition of CaCl2 promoted the fusion even further. The data are discussed with respect to the possibility of transformation of pollen by liposome-mediated introduction of nucleic acids into the pollen tube.

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Fusion of germinating watermelon pollen tubes with liposomes

Abstract

Keywords

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