Fusion of the promoter region of rRNA operon rrnB to lac Z gene

Gad Glaser; Simi Kobi; Amos B. Oppenheim

doi:10.1093/nar/8.19.4327

Fusion of the promoter region of rRNA operon rrnB to lac Z gene

Gad Glaser^*, Simi Kobi, Amos B. Oppenheim

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A Lambda phage was constructed in which the structural gene for β galactosidase is fused to a DNA segment carrying the ribosomal promoter rrnB of E. coli. In this hybrid operon β galactosidase synthesis in vitro is repressed by ppGpp. Repression of β galactosidase synthesis by cAMP is reported.

Original language	English
Pages (from-to)	4327-4336
Number of pages	10
Journal	Nucleic Acids Research
Volume	8
Issue number	19
DOIs	https://doi.org/10.1093/nar/8.19.4327
State	Published - 10 Oct 1980

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abstract = "A Lambda phage was constructed in which the structural gene for β galactosidase is fused to a DNA segment carrying the ribosomal promoter rrnB of E. coli. In this hybrid operon β galactosidase synthesis in vitro is repressed by ppGpp. Repression of β galactosidase synthesis by cAMP is reported.",

author = "Gad Glaser and Simi Kobi and Oppenheim, \{Amos B.\}",

year = "1980",

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AU - Glaser, Gad

AU - Kobi, Simi

AU - Oppenheim, Amos B.

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Y1 - 1980/10/10

N2 - A Lambda phage was constructed in which the structural gene for β galactosidase is fused to a DNA segment carrying the ribosomal promoter rrnB of E. coli. In this hybrid operon β galactosidase synthesis in vitro is repressed by ppGpp. Repression of β galactosidase synthesis by cAMP is reported.

AB - A Lambda phage was constructed in which the structural gene for β galactosidase is fused to a DNA segment carrying the ribosomal promoter rrnB of E. coli. In this hybrid operon β galactosidase synthesis in vitro is repressed by ppGpp. Repression of β galactosidase synthesis by cAMP is reported.

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JO - Nucleic Acids Research

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ER -

Fusion of the promoter region of rRNA operon rrnB to lac Z gene

Abstract

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