FUT2 genotype and secretory status are not associated with fecal microbial composition and inferred function in healthy subjects

Williams Turpin, Larbi Bedrani, Osvaldo Espin-Garcia, Wei Xu, Mark S. Silverberg, Michelle I. Smith, David S. Guttman, Anne Griffiths, Paul Moayyedi, Remo Panaccione, Hien Huynh, Hillary Steinhart, Guy Aumais, Konstantin Shestopaloff, Levinus A. Dieleman, Dan Turner, Andrew D. Paterson, Kenneth Croitoru*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Heritability analysis of the microbiota has demonstrated the importance of host genotype in defining the human microbiota. The alpha (1,2)-fucosyltransferase 2 encoded by FUT2 is involved in the formation of the H antigen and the SNP, rs601338 is associated with ABO histo-blood group antigen secretion in the intestinal mucosa. Previous studies have provided non replicated results for the association of this polymorphism with the composition and inferred function of intestinal microbiota. We aimed to assess this relationship in a large cohort of 1,190 healthy individuals. Genotyping was performed using the HumanCoreEXOME chip, microbial composition was addressed by 16S rRNA gene sequencing. Firmicutes, Bacteroidetes, and Actinobacteria were the dominant phyla in this cohort. Although we have sufficient power to detect significant associations of FUT2 genotype/ inferred phenotype with the microbiota, our data demonstrate that FUT2 genotype and secretor status is not associated with microbial alpha diversity, microbial composition or inferred microbial function after correction for multiple testing. Thus, FUT2 genotype and inferred phenotype are not associated with human fecal microbial composition and imputed function.

Original languageAmerican English
Pages (from-to)357-368
Number of pages12
JournalGut Microbes
Volume9
Issue number4
DOIs
StatePublished - 4 Jul 2018
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by grants from Crohn’s and Colitis Canada, Canadian Institutes of Health Research (CIHR) Grant #CMF108031 and the Helmsley Charitable Trust. Williams Turpin is a recipient of a Postdoctoral Fellowship Research Award from the CIHR Fellowship/ Canadian Association of Gastroenterology (CAG)/ Ferring Pharmaceuticals Inc. and a fellowship from the Department of Medicine, Mount Sinai Hospital, Toronto. Mark Silverberg is supported in part by the Gale and Graham Wright Chair in Digestive Diseases. David Kevans is a recipient of a CIHR /CAG/Abbvie IBD Fellowship Award. Osvaldo Espin-Garcia is a recipient of a CIHR STAGE fellowship. Konstantin Shestopaloff is a recipient of an Ontario Graduate Scholarship.

Funding Information:
This study was supported by grants from Crohn's and Colitis Canada, Canadian Institutes of Health Research (CIHR) Grant #CMF108031 and the Helmsley Charitable Trust. Williams Turpin is a recipient of a Postdoctoral Fellowship Research Award from the CIHR Fellowship/ Canadian Association of Gastroenterology (CAG)/ Ferring Pharmaceuticals Inc. and a fellowship from the Department of Medicine, Mount Sinai Hospital, Toronto. Mark Silverberg is supported in part by the Gale and Graham Wright Chair in Digestive Diseases. David Kevans is a recipient of a CIHR /CAG/Abbvie IBD Fellowship Award. Osvaldo Espin-Garcia is a recipient of a CIHR STAGE fellowship. Konstantin Shestopaloff is a recipient of an Ontario Graduate Scholarship. We thank the members of the GEM Global Project Office: Cristina Bravi, Deema Couchman, Nisha Ganeswaren, Alexandra Keludjian, Kevin Ow, Rachel Caplan, Melissa Greaves, Amy Craig-Neil, Ana Olteanu, Nellie Allam, Amanda Garrioch, Denis Ng, Venus Onay and Isabelle Yeadon for administrative support. We thank Dennis Cvitkovitch for his helpful scientific discussion of the project.

Publisher Copyright:
© 2018, © 2018 Taylor & Francis.

Keywords

  • ABO antigen
  • Blood group antigens
  • Fecal microbiota
  • Fucosylation
  • Gut microbiota
  • Healthy human
  • Microbiome
  • human genetic
  • mucus

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