TY - JOUR
T1 - G protein β2 subunit antisense oligonucleotides inhibit cell proliferation and disorganize microtubule and mitotic spindle organization
AU - Zou, Wei
AU - Hakim, Imad
AU - Tschoep, Katharina
AU - Endres, Stefan
AU - Bar-Shavit, Zvi
PY - 2001
Y1 - 2001
N2 - The association of G protein β2 subunit (Gβ2) with mitotic spindles in various mammalian cells has been demonstrated previously. Recently, we have identified the association of Gβ2 protein with microtubules (Wu et al., [1998] J. Cell. Biochem. 70: 552-562). In the present experiment we have demonstrated the possible functional role of Gβ2 in microtubule and mitotic spindle organization in mammalian cells. When Gβ2 antisense phosphorothioate oligonucleotides were transfected into mammalian cells, inhibition of cell proliferation with cell death after a 4-day treatment was observed. If the transfected cells were incubated for two days and their Gβ2 and microtubules were examined by Western blotting and immunofluorescence localization, marked reduction of the Gβ2 protein, fragmentation and disassembly of cytoplasmic microtubules, and disorganized mitotic spindles were found. We conclude that the Gβ2 protein is closely associated with microtubule assembly and may play a potential role in the regulation of cell proliferation and microtubule and mitotic spindle organization in mammalian cells.
AB - The association of G protein β2 subunit (Gβ2) with mitotic spindles in various mammalian cells has been demonstrated previously. Recently, we have identified the association of Gβ2 protein with microtubules (Wu et al., [1998] J. Cell. Biochem. 70: 552-562). In the present experiment we have demonstrated the possible functional role of Gβ2 in microtubule and mitotic spindle organization in mammalian cells. When Gβ2 antisense phosphorothioate oligonucleotides were transfected into mammalian cells, inhibition of cell proliferation with cell death after a 4-day treatment was observed. If the transfected cells were incubated for two days and their Gβ2 and microtubules were examined by Western blotting and immunofluorescence localization, marked reduction of the Gβ2 protein, fragmentation and disassembly of cytoplasmic microtubules, and disorganized mitotic spindles were found. We conclude that the Gβ2 protein is closely associated with microtubule assembly and may play a potential role in the regulation of cell proliferation and microtubule and mitotic spindle organization in mammalian cells.
KW - Antisense oligonucleotide transfection
KW - Gβ2 protein
KW - Microtubule assembly
KW - Mitotic spindle organization
KW - Nasopharyngeal carcinoma cells
UR - http://www.scopus.com/inward/record.url?scp=0034741644&partnerID=8YFLogxK
U2 - 10.1002/jcb.1210
DO - 10.1002/jcb.1210
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C2 - 11500962
AN - SCOPUS:0034741644
SN - 0730-2312
VL - 83
SP - 136
EP - 146
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
IS - 1
ER -