Gas chromatographic determination of novel valproyl taurinamide derivatives in mouse and dog plasma

Nina Isoherranen, Boris Yagen, Ofer Spiegelstein, Amir Steinman, Richard H. Finnell, Meir Bialer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Valproyl taurinamides are a novel group of compounds that possess anticonvulsant activity. In this study a gas chromatographic micromethod was developed for the quantification of selected valproyl taurinamides and some of their metabolites in biological samples. Valproyl taurinamide (VTD), N-methyl valproyl taurinamide (M-VTD), N,N-dimethyl valproyl taurinamide (DM-VTD) and N-isopropyl valproyl taurinamide (I-VTD) were analyzed in mouse and dog plasma and in dog urine using gas chromatography. Flame ionization detection and mass spectrometric detection were compared. The plasma samples were prepared by solid-phase extraction using C18 cartridges. The urine samples were prepared by liquid-liquid extraction. The sample volume used was 100 μl of dog plasma, 50 μl of mouse plasma and 20 μl of dog or mouse urine. The quantification range of the method was 1.5-50 mg/l in dog plasma (VTD only), 2.5-250 mg/l in mouse plasma (0.7-90 pmol injected) and 0.04-2 mg/ml in dog urine (VTD only). The inter-day precision in plasma and urine samples was around 10% for all quantified concentrations except LOQ (15-20%). The accuracy for all four compounds was between 90 and 110% within the entire concentration range. The developed method was suitable for quantification of a series of CNS-active valproyl taurineamide derivatives in biological samples at relevant in vivo concentrations.

Original languageAmerican English
Pages (from-to)125-136
Number of pages12
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Issue number1
StatePublished - 5 May 2003

Bibliographical note

Funding Information:
This study was supported in part by the Horowitz fund of the Hebrew University of Jerusalem, Israel.


  • Derivatization, GC
  • Valproyl taurinamide


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