Gene amplification in non-Hodgkin's lymphoma

D. Ben-Yehuda*, J. Houldsworth, N. Z. Parsa, R. S.K. Chaganti

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Among 426 consecutively ascertained and karyotypically abnormal non- Hodgkin's lymphoma (NHL) tumours, cytological evidence for gene amplification in the form of homogeneously staining regions (HSRs) was encountered in nine cases of large cell diffuse lymphoma (LC-DL). The mean age of patients with HSRs was 62.9 years and four died within a year of diagnosis. To identify candidate gene(s) amplified in these tumours, we performed a Southern blot analysis of tumour DNA using probes for 23 known proto-oncogenes and the multidrug resistance gene, PGY1. Besides a two-fold amplification of the BCL2 gene in two cases, no evidence for overt amplification of any of the genes assayed was found. To confirm DNA amplification in these specimens we performed the DNA in-gel renaturation assay. Evidence for presence of amplified DNA fragments was obtained in four of seven specimens. These results suggest amplification of a novel gene(s). To our knowledge, this is the first formal study of gene amplification in a large consecutively ascertained series of fresh lymphoma biopsies.

Original languageAmerican English
Pages (from-to)792-797
Number of pages6
JournalBritish Journal of Haematology
Volume86
Issue number4
DOIs
StatePublished - 1994
Externally publishedYes

Keywords

  • double minute chromosomes
  • gene amplification
  • homogeneously staining regions
  • non-Hodgkin's lymphoma

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