TY - JOUR
T1 - Gene amplification in non-Hodgkin's lymphoma
AU - Ben-Yehuda, D.
AU - Houldsworth, J.
AU - Parsa, N. Z.
AU - Chaganti, R. S.K.
PY - 1994
Y1 - 1994
N2 - Among 426 consecutively ascertained and karyotypically abnormal non- Hodgkin's lymphoma (NHL) tumours, cytological evidence for gene amplification in the form of homogeneously staining regions (HSRs) was encountered in nine cases of large cell diffuse lymphoma (LC-DL). The mean age of patients with HSRs was 62.9 years and four died within a year of diagnosis. To identify candidate gene(s) amplified in these tumours, we performed a Southern blot analysis of tumour DNA using probes for 23 known proto-oncogenes and the multidrug resistance gene, PGY1. Besides a two-fold amplification of the BCL2 gene in two cases, no evidence for overt amplification of any of the genes assayed was found. To confirm DNA amplification in these specimens we performed the DNA in-gel renaturation assay. Evidence for presence of amplified DNA fragments was obtained in four of seven specimens. These results suggest amplification of a novel gene(s). To our knowledge, this is the first formal study of gene amplification in a large consecutively ascertained series of fresh lymphoma biopsies.
AB - Among 426 consecutively ascertained and karyotypically abnormal non- Hodgkin's lymphoma (NHL) tumours, cytological evidence for gene amplification in the form of homogeneously staining regions (HSRs) was encountered in nine cases of large cell diffuse lymphoma (LC-DL). The mean age of patients with HSRs was 62.9 years and four died within a year of diagnosis. To identify candidate gene(s) amplified in these tumours, we performed a Southern blot analysis of tumour DNA using probes for 23 known proto-oncogenes and the multidrug resistance gene, PGY1. Besides a two-fold amplification of the BCL2 gene in two cases, no evidence for overt amplification of any of the genes assayed was found. To confirm DNA amplification in these specimens we performed the DNA in-gel renaturation assay. Evidence for presence of amplified DNA fragments was obtained in four of seven specimens. These results suggest amplification of a novel gene(s). To our knowledge, this is the first formal study of gene amplification in a large consecutively ascertained series of fresh lymphoma biopsies.
KW - double minute chromosomes
KW - gene amplification
KW - homogeneously staining regions
KW - non-Hodgkin's lymphoma
UR - http://www.scopus.com/inward/record.url?scp=0028337570&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2141.1994.tb04831.x
DO - 10.1111/j.1365-2141.1994.tb04831.x
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C2 - 7918074
AN - SCOPUS:0028337570
SN - 0007-1048
VL - 86
SP - 792
EP - 797
JO - British Journal of Haematology
JF - British Journal of Haematology
IS - 4
ER -
