TY - JOUR
T1 - Gene transfer by viral vectors into blood vessels in a rat model of retinopathy of prematurity
AU - Chowers, I.
AU - Banin, E.
AU - Hemo, Y.
AU - Porat, R.
AU - Falk, H.
AU - Keshet, E.
AU - Pe'er, J.
AU - Panet, A.
PY - 2001
Y1 - 2001
N2 - Aims - To test the feasibility of gene transfer into hyaloid blood vessels and into preretinal neovascularisation in a rat model of retinopathy of prematurity (RAP), using different viral vectors. Methods - Newborn rats were exposed to alternating hypoxic and hyperoxic conditions in order to induce ocular neovascularisation (ROP rats). Adenovirus, herpes simplex, vaccinia, and retroviral (MuLV based) vectors, all carrying the β galactosidase (β-gal) gene, were injected intravitreally on postnatal day 18 (P18). Two sets of controls were also examined: P18 ROP rats injected with saline and P18 rats that were raised in room air before the viral vectors or saline were injected. Two days after injection, the rats were killed, eyes enucleated, and β-gal expression was examined by X-gal staining in whole mounts and in histological sections. Results - Intravitreal injection of the adenovirus and vaccinia vectors yielded marked β-gal expression in hyaloid blood vessels in the rat ROP model. Retinal expression of β-gal with these vectors was limited almost exclusively to the vicinity of the injection site. Injection of herpes simplex yielded a punctuate pattern of β-gal expression in the retina but not in blood vessels. No significant β-gal expression occurred in rat eyes injected with the retroviral vector. Conclusions - Adenovirus is an efficient vector for gene transfer into blood vessels in an animal model of ROP. This may be a first step towards utilising gene transfer as a tool for modulating ocular neovascularisation for experimental and therapeutic purposes.
AB - Aims - To test the feasibility of gene transfer into hyaloid blood vessels and into preretinal neovascularisation in a rat model of retinopathy of prematurity (RAP), using different viral vectors. Methods - Newborn rats were exposed to alternating hypoxic and hyperoxic conditions in order to induce ocular neovascularisation (ROP rats). Adenovirus, herpes simplex, vaccinia, and retroviral (MuLV based) vectors, all carrying the β galactosidase (β-gal) gene, were injected intravitreally on postnatal day 18 (P18). Two sets of controls were also examined: P18 ROP rats injected with saline and P18 rats that were raised in room air before the viral vectors or saline were injected. Two days after injection, the rats were killed, eyes enucleated, and β-gal expression was examined by X-gal staining in whole mounts and in histological sections. Results - Intravitreal injection of the adenovirus and vaccinia vectors yielded marked β-gal expression in hyaloid blood vessels in the rat ROP model. Retinal expression of β-gal with these vectors was limited almost exclusively to the vicinity of the injection site. Injection of herpes simplex yielded a punctuate pattern of β-gal expression in the retina but not in blood vessels. No significant β-gal expression occurred in rat eyes injected with the retroviral vector. Conclusions - Adenovirus is an efficient vector for gene transfer into blood vessels in an animal model of ROP. This may be a first step towards utilising gene transfer as a tool for modulating ocular neovascularisation for experimental and therapeutic purposes.
UR - http://www.scopus.com/inward/record.url?scp=0034895201&partnerID=8YFLogxK
U2 - 10.1136/bjo.85.8.991
DO - 10.1136/bjo.85.8.991
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 11466260
AN - SCOPUS:0034895201
SN - 0007-1161
VL - 85
SP - 991
EP - 995
JO - British Journal of Ophthalmology
JF - British Journal of Ophthalmology
IS - 8
ER -