Gene transfer in plant protoplasts Inhibition of gene activity by cytosine methylation and expression of single-stranded DNA constructs

Maty Hershkovitz; Yosef Gruenbaum; Nehama Zakai; Abraham Loyter

doi:10.1016/0014-5793(89)80952-4

Gene transfer in plant protoplasts Inhibition of gene activity by cytosine methylation and expression of single-stranded DNA constructs

Maty Hershkovitz, Yosef Gruenbaum, Nehama Zakai, Abraham Loyter^*

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Transfection of petunia protoplasts with the plasmid pUC118CaMVCAT, bearing the bacterial chloramphenicol acetyltransferase (CAT) gene, resulted in the appearance of CAT activity. No CAT activity was detected in protoplasts transfected with the hemimethylated form of pUC118CaMVCAT. Transfection of petunia protoplasts with ss DNA constructs bearing the coding and noncoding sequences of the CAT gene also resulted in the appearance of CAT activity, indicating expression of ss DNA constructs in transfected protoplasts. The relevance of our observations to control of gene expression in plant cells is discussed.

Original language	English
Pages (from-to)	167-172
Number of pages	6
Journal	FEBS Letters
Volume	253
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(89)80952-4
State	Published - 14 Aug 1989

Keywords

DNA methylation
Gene regulation
Plant protoplast

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title = "Gene transfer in plant protoplasts Inhibition of gene activity by cytosine methylation and expression of single-stranded DNA constructs",

abstract = "Transfection of petunia protoplasts with the plasmid pUC118CaMVCAT, bearing the bacterial chloramphenicol acetyltransferase (CAT) gene, resulted in the appearance of CAT activity. No CAT activity was detected in protoplasts transfected with the hemimethylated form of pUC118CaMVCAT. Transfection of petunia protoplasts with ss DNA constructs bearing the coding and noncoding sequences of the CAT gene also resulted in the appearance of CAT activity, indicating expression of ss DNA constructs in transfected protoplasts. The relevance of our observations to control of gene expression in plant cells is discussed.",

keywords = "DNA methylation, Gene regulation, Plant protoplast",

author = "Maty Hershkovitz and Yosef Gruenbaum and Nehama Zakai and Abraham Loyter",

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AU - Gruenbaum, Yosef

AU - Zakai, Nehama

AU - Loyter, Abraham

PY - 1989/8/14

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N2 - Transfection of petunia protoplasts with the plasmid pUC118CaMVCAT, bearing the bacterial chloramphenicol acetyltransferase (CAT) gene, resulted in the appearance of CAT activity. No CAT activity was detected in protoplasts transfected with the hemimethylated form of pUC118CaMVCAT. Transfection of petunia protoplasts with ss DNA constructs bearing the coding and noncoding sequences of the CAT gene also resulted in the appearance of CAT activity, indicating expression of ss DNA constructs in transfected protoplasts. The relevance of our observations to control of gene expression in plant cells is discussed.

AB - Transfection of petunia protoplasts with the plasmid pUC118CaMVCAT, bearing the bacterial chloramphenicol acetyltransferase (CAT) gene, resulted in the appearance of CAT activity. No CAT activity was detected in protoplasts transfected with the hemimethylated form of pUC118CaMVCAT. Transfection of petunia protoplasts with ss DNA constructs bearing the coding and noncoding sequences of the CAT gene also resulted in the appearance of CAT activity, indicating expression of ss DNA constructs in transfected protoplasts. The relevance of our observations to control of gene expression in plant cells is discussed.

KW - DNA methylation

KW - Gene regulation

KW - Plant protoplast

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Gene transfer in plant protoplasts Inhibition of gene activity by cytosine methylation and expression of single-stranded DNA constructs

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Keywords

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