TY - JOUR
T1 - Gene transfer into the lung by nanoparticle dextran-spermine/plasmid DNA complexes
AU - Abdullah, Syahril
AU - Wendy-Yeo, Wai Yeng
AU - Hosseinkhani, Hossein
AU - Hosseinkhani, Mohsen
AU - Masrawa, Ehab
AU - Ramasamy, Rajesh
AU - Rosli, Rozita
AU - Rahman, Sabariah A.
AU - Domb, Abraham J.
PY - 2010
Y1 - 2010
N2 - A novel cationic polymer, dextran-spermine (D-SPM), has been found to mediate gene expression in a wide variety of cell lines and in vivo through systemic delivery. Here, we extended the observations by determining the optimal conditions for gene expression of D-SPM/plasmid DNA (D-SPM/pDNA) in cell lines and in the lungs of BALB/c mice via instillation delivery. In vitro studies showed that D-SPM could partially protect pDNA from degradation by nuclease and exhibited optimal gene transfer efficiency at D-SPM to pDNA weight-mixing ratio of 12. In the lungs of mice, the levels of gene expression generated by D-SPM/pDNA are highly dependent on the weight-mixing ratio of D-SPM to pDNA, amount of pDNA in the complex, and the assay time postdelivery. Readministration of the complex at day 1 following the first dosing showed no significant effect on the retention and duration of gene expression. The study also showed that there was a clear trend of increasing size of the complexes as the amount of pDNA was increased, where the sizes of the D-SPM/pDNA complexes were within the nanometer range.
AB - A novel cationic polymer, dextran-spermine (D-SPM), has been found to mediate gene expression in a wide variety of cell lines and in vivo through systemic delivery. Here, we extended the observations by determining the optimal conditions for gene expression of D-SPM/plasmid DNA (D-SPM/pDNA) in cell lines and in the lungs of BALB/c mice via instillation delivery. In vitro studies showed that D-SPM could partially protect pDNA from degradation by nuclease and exhibited optimal gene transfer efficiency at D-SPM to pDNA weight-mixing ratio of 12. In the lungs of mice, the levels of gene expression generated by D-SPM/pDNA are highly dependent on the weight-mixing ratio of D-SPM to pDNA, amount of pDNA in the complex, and the assay time postdelivery. Readministration of the complex at day 1 following the first dosing showed no significant effect on the retention and duration of gene expression. The study also showed that there was a clear trend of increasing size of the complexes as the amount of pDNA was increased, where the sizes of the D-SPM/pDNA complexes were within the nanometer range.
UR - http://www.scopus.com/inward/record.url?scp=77955405118&partnerID=8YFLogxK
U2 - 10.1155/2010/284840
DO - 10.1155/2010/284840
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C2 - 20617146
AN - SCOPUS:77955405118
SN - 1110-7243
VL - 2010
JO - Journal of Biomedicine and Biotechnology
JF - Journal of Biomedicine and Biotechnology
M1 - 284840
ER -