Generation and characterization of a mouse model for one anastomosis gastric bypass surgery

Samir Abu-Gazala, Michael Bergel, Yhara Arad, Liron Hefetz, Shira Azulai, Aaron Baker, Arnon Haran, Hadar Israeli, Doron Kleiman, Itia Samuel, Uria Tsubary, Anna Permyakova, Joseph Tam, Rachel Ben-Haroush Schyr, Danny Ben-Zvi

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

One anastomosis gastric bypass (OAGB) surgery became a common bariatric procedure in recent years. In this surgery, the distal stomach, duodenum, and proximal jejunum are bypassed, leading to weight loss, improvement in metabolic parameters, and a change in hormonal secretion. We sought to generate and characterize a mouse model for OAGB. Mice fed for 26 wk on a high-fat diet were assigned to OAGB, sham surgery, or caloric restriction and were followed for 50 more days on a high-fat diet. Physiological and histological parameters of the mice were compared during and at the end of the experiment. OAGB-operated mice lost weight and displayed low levels of plasma lipids, high insulin sensitivity, and rapid glucose metabolism compared with sham-operated mice. OAGB-operated mice had higher energy expenditure, higher levels of glucagon-like peptide (GLP-1), and lower albumin than weight-matched calorie-restricted mice. There was no difference in the histology of the endocrine pancreas. The livers of OAGB mice had little hepatic steatosis yet presented with a large number of phagocytic cells. The OAGB mouse model recapitulates many of the phenotypes described in patients that underwent OAGB and enables molecular and physiological studies on the outcome of this surgery. NEW & NOTEWORTHY A mouse model for one anastomosis gastric bypass (OAGB) surgery displays similar outcomes to clinical reports and enables to study the weight loss-dependent and -independent effects of this bariatric surgery.

Original languageAmerican English
Pages (from-to)E414-E424
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume322
Issue number5
DOIs
StatePublished - May 2022

Bibliographical note

Publisher Copyright:
Copyright © 2022 the American Physiological Society.

Keywords

  • bariatric surgery
  • mouse model
  • obesity
  • Humans
  • Insulin Resistance
  • Obesity, Morbid/metabolism
  • Animals
  • Gastric Bypass/methods
  • Bariatric Surgery/methods
  • Mice
  • Retrospective Studies
  • Weight Loss/physiology
  • Disease Models, Animal

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