Genetic analysis of the origin of plants regenerated from anther tissues of Lycopersicon esculentum mill

Anther cultures of a male-sterile tomato mutant (ms 10³⁵) which produce callus and regenerate plants were investigated as a potential source of haploids. To facilitate this study, male-sterile plants were constructed which were heterozygous for morphological and enzymic markers. None of the regenerated plants were hemizygous for the genetic markers, which together with chromosome counts, demonstrated that the regenerates analyzed originated entirely from maternal diploid cells. The isozyme technique is discussed as a means of facilitating the detection of plantlets derived from haploid tissue.