Genetic and morphologic differentiation of Bolbophorus confusus and B. levantinus (Digenea: Diplostomatidae), based on rDNA SSU polymorphism and SEM

R. Dzikowski*, M. G. Levy, M. F. Poore, J. R. Flowers, I. Paperna

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Metacercariae of Bolbophorus species are serious pathogens of farmed fish. Molecular diagnostic tools, capable of identifying and differentiating these parasites, may assist in the development of rationale control strategies. The rDNA 18S (small sub-unit: SSU) genes of adult B. confusus and B. levantinus obtained from a pelican, Pelecanus onocrotalus, and a night heron, Nycticorax nycticorax, respectively, were amplified, sequenced, and aligned. Based on this alignment, we developed a genetic differentiation assay between B. confusus and B. levantinus. These 2 species were compared genetically with the North American species B. damnificus and Bolbophorus sp. (Type 2'). The relationship between species is outlined and discussed. In addition to the molecular study, specimens of B. confusus and B. levantinus were compared morphologically, using scanning electron microscopy. Morphologic analysis revealed interspecific differences in details of the holdfast organ and the position of the acetabulum.

Original languageAmerican English
Pages (from-to)231-235
Number of pages5
JournalDiseases of Aquatic Organisms
Volume57
Issue number3
DOIs
StatePublished - 29 Dec 2003

Keywords

  • 18S (SSU) rDNA gene
  • Bolbophorus confusus
  • Bolobphorus levantinus
  • Digenea
  • Diplostomatidae
  • SEM

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