Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis

Abhaypratap Vishwakarma, Ahan Dalal, Sarada Devi Tetali, Pulugurtha Bharadwaja Kirti, Kollipara Padmasree*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (AtAOX1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX1a transformant (pYES2AtAOX1a) showed cyanide resistant and salicylhydroxamic acid (SHAM)-sensitive respiration, indicating functional expression of AtAOX1a in S. cerevisiae. After exposure to oxidative stress, pYES2AtAOX1a showed better survival and a decrease in reactive oxygen species (ROS) when compared to S. cerevisiae with empty vector (pYES2). Furthermore, pYES2AtAOX1a sustained growth by regulating GPX2 and/or TSA2, and cellular NAD+/NADH ratio. Thus, the expression of AtAOX1a in S. cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage.

Original languageAmerican English
Pages (from-to)135-146
Number of pages12
JournalFEBS Open Bio
Volume6
Issue number2
DOIs
StatePublished - 1 Feb 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016 Authors.

Keywords

  • Alternative oxidase 1a
  • Oxidative stress
  • Reactive oxygen species
  • Redox homeostasis
  • Respiration
  • Saccharomyces cerevisiae

Fingerprint

Dive into the research topics of 'Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis'. Together they form a unique fingerprint.

Cite this