TY - JOUR
T1 - Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis
AU - Vishwakarma, Abhaypratap
AU - Dalal, Ahan
AU - Tetali, Sarada Devi
AU - Kirti, Pulugurtha Bharadwaja
AU - Padmasree, Kollipara
N1 - Publisher Copyright:
© 2016 Authors.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (AtAOX1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX1a transformant (pYES2AtAOX1a) showed cyanide resistant and salicylhydroxamic acid (SHAM)-sensitive respiration, indicating functional expression of AtAOX1a in S. cerevisiae. After exposure to oxidative stress, pYES2AtAOX1a showed better survival and a decrease in reactive oxygen species (ROS) when compared to S. cerevisiae with empty vector (pYES2). Furthermore, pYES2AtAOX1a sustained growth by regulating GPX2 and/or TSA2, and cellular NAD+/NADH ratio. Thus, the expression of AtAOX1a in S. cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage.
AB - This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (AtAOX1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX1a transformant (pYES2AtAOX1a) showed cyanide resistant and salicylhydroxamic acid (SHAM)-sensitive respiration, indicating functional expression of AtAOX1a in S. cerevisiae. After exposure to oxidative stress, pYES2AtAOX1a showed better survival and a decrease in reactive oxygen species (ROS) when compared to S. cerevisiae with empty vector (pYES2). Furthermore, pYES2AtAOX1a sustained growth by regulating GPX2 and/or TSA2, and cellular NAD+/NADH ratio. Thus, the expression of AtAOX1a in S. cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage.
KW - Alternative oxidase 1a
KW - Oxidative stress
KW - Reactive oxygen species
KW - Redox homeostasis
KW - Respiration
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=84958743872&partnerID=8YFLogxK
U2 - 10.1002/2211-5463.12028
DO - 10.1002/2211-5463.12028
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AN - SCOPUS:84958743872
SN - 2211-5463
VL - 6
SP - 135
EP - 146
JO - FEBS Open Bio
JF - FEBS Open Bio
IS - 2
ER -