Abstract
This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (AtAOX1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX1a transformant (pYES2AtAOX1a) showed cyanide resistant and salicylhydroxamic acid (SHAM)-sensitive respiration, indicating functional expression of AtAOX1a in S. cerevisiae. After exposure to oxidative stress, pYES2AtAOX1a showed better survival and a decrease in reactive oxygen species (ROS) when compared to S. cerevisiae with empty vector (pYES2). Furthermore, pYES2AtAOX1a sustained growth by regulating GPX2 and/or TSA2, and cellular NAD+/NADH ratio. Thus, the expression of AtAOX1a in S. cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage.
Original language | American English |
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Pages (from-to) | 135-146 |
Number of pages | 12 |
Journal | FEBS Open Bio |
Volume | 6 |
Issue number | 2 |
DOIs | |
State | Published - 1 Feb 2016 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2016 Authors.
Keywords
- Alternative oxidase 1a
- Oxidative stress
- Reactive oxygen species
- Redox homeostasis
- Respiration
- Saccharomyces cerevisiae