Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis

Abhaypratap Vishwakarma; Ahan Dalal; Sarada Devi Tetali; Pulugurtha Bharadwaja Kirti; Kollipara Padmasree

doi:10.1002/2211-5463.12028

Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis

Abhaypratap Vishwakarma, Ahan Dalal, Sarada Devi Tetali, Pulugurtha Bharadwaja Kirti, Kollipara Padmasree^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (AtAOX1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX1a transformant (pYES2AtAOX1a) showed cyanide resistant and salicylhydroxamic acid (SHAM)-sensitive respiration, indicating functional expression of AtAOX1a in S. cerevisiae. After exposure to oxidative stress, pYES2AtAOX1a showed better survival and a decrease in reactive oxygen species (ROS) when compared to S. cerevisiae with empty vector (pYES2). Furthermore, pYES2AtAOX1a sustained growth by regulating GPX2 and/or TSA2, and cellular NAD⁺/NADH ratio. Thus, the expression of AtAOX1a in S. cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage.

Original language	American English
Pages (from-to)	135-146
Number of pages	12
Journal	FEBS Open Bio
Volume	6
Issue number	2
DOIs	https://doi.org/10.1002/2211-5463.12028
State	Published - 1 Feb 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2016 Authors.

Keywords

Alternative oxidase 1a
Oxidative stress
Reactive oxygen species
Redox homeostasis
Respiration
Saccharomyces cerevisiae

Access to Document

10.1002/2211-5463.12028

Cite this

@article{4c7d03c3998141359f66a3510dba84cc,

title = "Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis",

abstract = "This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (AtAOX1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX1a transformant (pYES2AtAOX1a) showed cyanide resistant and salicylhydroxamic acid (SHAM)-sensitive respiration, indicating functional expression of AtAOX1a in S. cerevisiae. After exposure to oxidative stress, pYES2AtAOX1a showed better survival and a decrease in reactive oxygen species (ROS) when compared to S. cerevisiae with empty vector (pYES2). Furthermore, pYES2AtAOX1a sustained growth by regulating GPX2 and/or TSA2, and cellular NAD+/NADH ratio. Thus, the expression of AtAOX1a in S. cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage.",

keywords = "Alternative oxidase 1a, Oxidative stress, Reactive oxygen species, Redox homeostasis, Respiration, Saccharomyces cerevisiae",

author = "Abhaypratap Vishwakarma and Ahan Dalal and Tetali, {Sarada Devi} and Kirti, {Pulugurtha Bharadwaja} and Kollipara Padmasree",

note = "Publisher Copyright: {\textcopyright} 2016 Authors.",

year = "2016",

month = feb,

day = "1",

doi = "10.1002/2211-5463.12028",

language = "American English",

volume = "6",

pages = "135--146",

journal = "FEBS Open Bio",

issn = "2211-5463",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis

AU - Vishwakarma, Abhaypratap

AU - Dalal, Ahan

AU - Tetali, Sarada Devi

AU - Kirti, Pulugurtha Bharadwaja

AU - Padmasree, Kollipara

PY - 2016/2/1

Y1 - 2016/2/1

N2 - This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (AtAOX1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX1a transformant (pYES2AtAOX1a) showed cyanide resistant and salicylhydroxamic acid (SHAM)-sensitive respiration, indicating functional expression of AtAOX1a in S. cerevisiae. After exposure to oxidative stress, pYES2AtAOX1a showed better survival and a decrease in reactive oxygen species (ROS) when compared to S. cerevisiae with empty vector (pYES2). Furthermore, pYES2AtAOX1a sustained growth by regulating GPX2 and/or TSA2, and cellular NAD+/NADH ratio. Thus, the expression of AtAOX1a in S. cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage.

AB - This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (AtAOX1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX1a transformant (pYES2AtAOX1a) showed cyanide resistant and salicylhydroxamic acid (SHAM)-sensitive respiration, indicating functional expression of AtAOX1a in S. cerevisiae. After exposure to oxidative stress, pYES2AtAOX1a showed better survival and a decrease in reactive oxygen species (ROS) when compared to S. cerevisiae with empty vector (pYES2). Furthermore, pYES2AtAOX1a sustained growth by regulating GPX2 and/or TSA2, and cellular NAD+/NADH ratio. Thus, the expression of AtAOX1a in S. cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage.

KW - Alternative oxidase 1a

KW - Oxidative stress

KW - Reactive oxygen species

KW - Redox homeostasis

KW - Respiration

KW - Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=84958743872&partnerID=8YFLogxK

U2 - 10.1002/2211-5463.12028

DO - 10.1002/2211-5463.12028

M3 - Article

AN - SCOPUS:84958743872

SN - 2211-5463

VL - 6

SP - 135

EP - 146

JO - FEBS Open Bio

JF - FEBS Open Bio

IS - 2

ER -

Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this