Abstract
This chapter focuses on recent advances in the genetic manipulation of human embryonic stem (hES) cells, the available methods of manipulation, and their possible uses. A brief description of advanced genetic manipulation techniques, not yet demonstrated in these cells, is also presented. Genetic manipulation is the process of inducing changes in gene expression, and the expression of novel genes has proved to be an indispensable tool in recent genetic research. The implementation of increasingly powerful genetic tools for mouse embryonic stem (ES) cells has led to an explosion of data concerning the specific properties of an enormous array of genes. Genetic manipulation will play a pivotal role in applying this technology to biological research as well as to the specific needs of transplantation medicine. Several gene delivery methods have been shown to be effective in human ES cells including transfection by various chemical reagents, electro oration, and viral infection.
| Original language | English |
|---|---|
| Title of host publication | Embryonic |
| Publisher | Elsevier Inc. |
| Pages | 543-550 |
| Number of pages | 8 |
| Volume | 1 |
| ISBN (Electronic) | 9780080533735 |
| ISBN (Print) | 9780124366435 |
| DOIs | |
| State | Published - 14 Sep 2004 |
Bibliographical note
Publisher Copyright:© 2004 Elsevier Inc. All rights reserved.