TY - JOUR
T1 - Genetic mapping of tomato cDNA clones encoding the chloroplastic and the cytosolic isozymes of superoxide dismutase
AU - Perl-Treves, Rafael
AU - Abu-Abied, Mohamad
AU - Magal, Nurit
AU - Galun, Esra
AU - Zamir, Daniel
PY - 1990/10
Y1 - 1990/10
N2 - The isozyme pattern of superoxide dismutase (SOD) in tomato consists of two Cu,Zn isozymes located, respectively, in the chloroplast and in the cytosol, as well as additional isozymes of the Mn or Fe SOD type. We have shown that SOD-1 is the chloroplastic Cu,Zn SOD and is related to cDNA clone T10. Restriction fragment length polymorphism (RFLP) analysis was performed with two cDNA clones representing tomato Cu,Zn-superoxide dismutases. T10, coding for the chloroplast isozyme, was thus mapped to chromosome 11, between marker TG46 and TG108, while clone P31, coding for the cytosolic Cu,Zn SOD isozyme, was mapped to chromosome 1 between TG24 and TG81. SOD is associated with the response of plants to various environmental stresses; the mapping information presented here would permit the demonstration of this association by genetic analysis.
AB - The isozyme pattern of superoxide dismutase (SOD) in tomato consists of two Cu,Zn isozymes located, respectively, in the chloroplast and in the cytosol, as well as additional isozymes of the Mn or Fe SOD type. We have shown that SOD-1 is the chloroplastic Cu,Zn SOD and is related to cDNA clone T10. Restriction fragment length polymorphism (RFLP) analysis was performed with two cDNA clones representing tomato Cu,Zn-superoxide dismutases. T10, coding for the chloroplast isozyme, was thus mapped to chromosome 11, between marker TG46 and TG108, while clone P31, coding for the cytosolic Cu,Zn SOD isozyme, was mapped to chromosome 1 between TG24 and TG81. SOD is associated with the response of plants to various environmental stresses; the mapping information presented here would permit the demonstration of this association by genetic analysis.
KW - isozymes
KW - Lycopersicon
KW - restriction fragment length polymorphism (RFLP) map
KW - superoxide dismutase
UR - http://www.scopus.com/inward/record.url?scp=0025204592&partnerID=8YFLogxK
U2 - 10.1007/BF00554381
DO - 10.1007/BF00554381
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C2 - 2085316
AN - SCOPUS:0025204592
SN - 0006-2928
VL - 28
SP - 543
EP - 552
JO - Biochemical Genetics
JF - Biochemical Genetics
IS - 9-10
ER -