Genetic variation among Mycoplasma agalactiae isolates detected by the variant surface lipoprotein gene (vspA) of Mycoplasma bovis

Ravenna Flitman-Tene, Sharon Levisohn, Ricardo Rosenbusch, Eitan Rapoport, David Yogev*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Multiple restriction fragments, homologous to the previously described Mycoplasma bovis vspA gene, were identified in the chromosome of Mycoplasma agalactiae. The vspA, a representative variable surface lipoprotein gene of the vsp gene family, and four synthetic oligonucleotides, representing sequences complementary to selected regions of the vsp genes, were used as probes against digested chromosomal DNAs of several M. agalactiae clinical isolates. The resulting Southern blot analysis demonstrated a marked DNA polymorphism of multiple vspA-related fragments among the isolates. An oligonucleotide representing a conserved 5'-region common to all known vsp genes, was found to hybridize to multiple M. agalactiae genomic fragments while the other three oligonucleotides, representing distinct repetitive structures within the coding region of three known vsp genes (vspA, vspE, and vspF), failed to react. These results argue for the possible existence of a gene family in M. agalactiae analogous to the vsp system of M. bovis but comprised of diverse genes.

Original languageAmerican English
Pages (from-to)123-128
Number of pages6
JournalFEMS Microbiology Letters
Volume156
Issue number1
DOIs
StatePublished - 1 Nov 1997
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by a grant (IS-2540-95R) from the United States-Israel Binational Agricultural Research and Development Fund (BARD). The skilled assistance of N. Hatib in isolation of M. agalactiae from clinical samples is greatly appreciated.

Keywords

  • Gene family
  • Genetic variation
  • Mycoplasma agalactiae
  • Mycoplasma bovis
  • Variable surface lipoprotein

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